ARHGAP21 protein, a new partner of α-tubulin involved in cell-cell adhesion formation and essential for epithelial-mesenchymal transition
| Autor: | Davis M. Staley, Karin Spat Albino Barcellos, Mariana Lazarini, Jarom Y. Chung, Karla Priscila Vieira, João Agostinho Machado-Neto, Sara Teresinha Olalla Saad, Marc D.H. Hansen, Mark V. Wagner, Steven G. Call, Carolina L. Bigarella, Peter R. Langford |
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| Rok vydání: | 2012 |
| Předmět: |
Epithelial-Mesenchymal Transition
Time Factors Role of cell adhesions in neural development Cellular differentiation CDC42 Cell Communication Biology Biochemistry Epithelium Madin Darby Canine Kidney Cells Dogs Cell Movement Tubulin Cell Line Tumor Cell Adhesion Animals Humans Epithelial–mesenchymal transition Neoplasm Metastasis Cell adhesion cdc42 GTP-Binding Protein Molecular Biology GTPase-Activating Proteins Cell migration Acetylation Cell Biology Cell biology Cdc42 GTP-Binding Protein Cytoplasm RNA Interference |
| Zdroj: | The Journal of biological chemistry. 288(4) |
| ISSN: | 1083-351X |
| Popis: | Cell-cell adhesions and the cytoskeletons play important and coordinated roles in cell biology, including cell differentiation, development, and migration. Adhesion and cytoskeletal dynamics are regulated by Rho-GTPases. ARHGAP21 is a negative regulator of Rho-GTPases, particularly Cdc42. Here we assess the function of ARHGAP21 in cell-cell adhesion, cell migration, and scattering. We find that ARHGAP21 is localized in the nucleus, cytoplasm, or perinuclear region but is transiently redistributed to cell-cell junctions 4 h after initiation of cell-cell adhesion. ARHGAP21 interacts with Cdc42, and decreased Cdc42 activity coincides with the appearance of ARHGAP21 at the cell-cell junctions. Cells lacking ARHGAP21 expression show weaker cell-cell adhesions, increased cell migration, and a diminished ability to undergo hepatocyte growth factor-induced epithelial-mesenchymal transition (EMT). In addition, ARHGAP21 interacts with α-tubulin, and it is essential for α-tubulin acetylation in EMT. Our findings indicate that ARHGAP21 is a Rho-GAP involved in cell-cell junction remodeling and that ARHGAP21 affects migration and EMT through α-tubulin interaction and acetylation. |
| Databáze: | OpenAIRE |
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