Camelina sativa meal hydrolysate as sustainable biomass for the production of carotenoids by Rhodosporidium toruloides

Autor: Danilo Porro, Stefano Bertacchi, Maurizio Bettiga, Paola Branduardi
Přispěvatelé: Bertacchi, S, Bettiga, M, Porro, D, Branduardi, P
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
Renewable resources
lcsh:Biotechnology
Camelina sativa
Rhodosporidium toruloides
Management
Monitoring
Policy and Law
Raw material
01 natural sciences
Applied Microbiology and Biotechnology
Hydrolysate
lcsh:Fuel
03 medical and health sciences
lcsh:TP315-360
010608 biotechnology
Enzymatic hydrolysis
lcsh:TP248.13-248.65
Bio-based products
Renewable resources
Biorefinery
Camelina meal
Enzymatic hydrolysis
Rhodosporidium toruloides
Carotenoids
Separate hydrolysis and fermentation (SHF)
Simultaneous saccharification and fermentation (SSF)
Food science
030304 developmental biology
0303 health sciences
Camelina meal
biology
Renewable Energy
Sustainability and the Environment
Chemistry
Research
food and beverages
biology.organism_classification
Biorefinery
Carotenoids
Bio-based products
CHIM/11 - CHIMICA E BIOTECNOLOGIA DELLE FERMENTAZIONI
Camelina
General Energy
Separate hydrolysis and fermentation (SHF)
Energy source
Simultaneous saccharification and fermentation (SSF)
Biotechnology
Zdroj: Biotechnology for Biofuels, Vol 13, Iss 1, Pp 1-10 (2020)
Biotechnology for Biofuels
Popis: Background As the circular economy advocates a near total waste reduction, the industry has shown an increased interest toward the exploitation of various residual biomasses. The origin and availability of biomass used as feedstock strongly affect the sustainability of biorefineries, where it is converted in energy and chemicals. Here, we explored the valorization of Camelina meal, the leftover residue from Camelina sativa oil extraction. In fact, in addition to Camelina meal use as animal feed, there is an increasing interest in further valorizing its macromolecular content or its nutritional value. Results Camelina meal hydrolysates were used as nutrient and energy sources for the fermentation of the carotenoid-producing yeast Rhodosporidium toruloides in shake flasks. Total acid hydrolysis revealed that carbohydrates accounted for a maximum of 31 ± 1.0% of Camelina meal. However, because acid hydrolysis is not optimal for subsequent microbial fermentation, an enzymatic hydrolysis protocol was assessed, yielding a maximum sugar recovery of 53.3%. Separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF), and SSF preceded by presaccharification of Camelina meal hydrolysate produced 5 ± 0.7, 16 ± 1.9, and 13 ± 2.6 mg/L of carotenoids, respectively. Importantly, the presence of water-insoluble solids, which normally inhibit microbial growth, correlated with a higher titer of carotenoids, suggesting that the latter could act as scavengers. Conclusions This study paves the way for the exploitation of Camelina meal as feedstock in biorefinery processes. The process under development provides an example of how different final products can be obtained from this side stream, such as pure carotenoids and carotenoid-enriched Camelina meal, can potentially increase the initial value of the source material. The obtained data will help assess the feasibility of using Camelina meal to generate high value-added products.
Databáze: OpenAIRE
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