Cytokines in systemic lupus erythematosus: far beyond Th1/Th2 dualism lupus: cytokine profiles
| Autor: | Lorena Flor da Rosa Franchi Santos, Poliana Macedo Guimarães, Edna Maria Vissoci Reiche, Andréa Name Colado Simão, Tatiana Mayumi Veiga Iriyoda, Neide Tomimura Costa, Marcell Alysson Batisti Lozovoy, Isaias Dichi, Bruna Miglioranza Scavuzzi, Nicole Perugini Stadtlober, Michael Maes |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_treatment Immunology T-Lymphocytes Regulatory 03 medical and health sciences 0302 clinical medicine Th2 Cells immune system diseases Dualism medicine Immunology and Allergy Humans Lupus Erythematosus Systemic Vitamin D skin and connective tissue diseases Th1-Th2 Balance 030203 arthritis & rheumatology B-Lymphocytes Systemic lupus erythematosus business.industry Gene Expression Profiling Cell Differentiation Cell Biology Middle Aged Th1 Cells medicine.disease Prognosis 030104 developmental biology Cytokine Case-Control Studies Cytokines Th17 Cells Female business |
| Zdroj: | Immunology and cell biology. 95(9) |
| ISSN: | 1440-1711 |
| Popis: | The aims of this study were to delineate cytokine profiles of systemic lupus erythematosus (SLE), construct prediction models for diagnosis and disease activity using those profiles, and to examine the associations between TNFB Ncol polymorphism, body mass index (BMI) and vitamin D levels with cytokine levels. Two hundred SLE patients and 196 healthy controls participated in this case-control study. Plasma cytokines levels of tumor necrosis factor (TNF)-α, interferon (IFN)-γ, interleukin (IL)-1β, IL- 4, IL-6, IL-10, IL-12 and IL-17 were measured and cytokines profiles were computed. IL-6, IL-12, IL-17, IFN-γ and IL-10 levels were significantly higher in SLE, while IL-4 was lower in SLE. The Th1/Th2 and Th1+Th17/Th2 profiles were significantly higher in SLE than in healthy controls, whereas there were no significant differences in the proinflammatory cytokine profile (TNFα+IL-6+IL-1β). In total, 90.4% of all subjects were correctly classified using Th1+Th17 profile and IL-10 (positively associated) and IL-4 (negatively associated) as predictor variables (sensitivity=66.7% and specificity=96.9%). In all, 20.9% of the variance in the SLE Disease Activity Index was predicted by the Th1+Th17/Th2 ratio, IL-10 and BMI (all positively) and proinflammatory profile (inversely associated). B1/B1 genotype is accompanied by increased IL-17 and Th17/Th2 ratio, while B1/B2 genotype is accompanied by higher IL-4 and IFNγ values. 25-OH vitamin D was inversely associated with IFN-γ levels. SLE is accompanied by Th1, Th17 and Treg profile and lowered IL-4 production. Lowered vitamin D levels and B1/B1 genotype, but not BMI, contribute to changes in cytokines profiles. Future treatments should target Th1, Th2 and Th17 profiles rather than inflammatory cytokines. |
| Databáze: | OpenAIRE |
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