Aromatic amino-acid biosynthesis in Candida albicans: identification of the ARO4 gene encoding a second DAHP synthase
| Autor: | George P. Livi, Sarita A. Pereira |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Mutant
Genes Fungal Molecular Sequence Data DAHP synthase Isozyme Polymerase Chain Reaction Homology (biology) chemistry.chemical_compound Candida albicans Genetics Aromatic amino acids 3-Deoxy-7-Phosphoheptulonate Synthase Amino Acid Sequence Amino Acids Gene Conserved Sequence Sequence Deletion biology Base Sequence Sequence Homology Amino Acid Nucleic acid sequence General Medicine biology.organism_classification Molecular biology Isoenzymes chemistry Biochemistry Mutagenesis biology.protein |
| Zdroj: | Current genetics. 29(5) |
| ISSN: | 0172-8083 |
| Popis: | The primary step in the aromatic amino-acid biosynthetic pathway in Saccharomyces cerevisiae is catalyzed by two redundant isozymes of 3-deoxy-d-arabinoheptulosonate-7-phosphate (DAHP) synthase, either of which alone is sufficient to permit growth on synthetic complete media lacking aromatic acids (SC-Aro). The activity of one isozyme (encoded by the ARO3 gene) is feedback-inhibited by phenylalanine, whereas the activity of the other isozyme (encoded by the ARO4 gene) is feedback-inhibited by tyrosine. Transcription of both genes is controlled by GCN4. We previously cloned the ARO3 gene from the opportunistic pathogen Candida albicans and found that: (1) it can complement an aro3 aro4 double mutation in S. cerevisiae, an effect inhibited by excess phenylalanine; and (2) its expression is induced in response to amino-acid deprivation, consistent with the presence of two putative GCN4-responsive promoter elements (Pereira and Livi 1993, 1995). To determine whether other DAHP synthases exist in C. albicans, we have constructed a homozygous aro3-deletion mutant strain. Such a mutant was found to be phenotypically Aro+, i. e., capable of normal growth on SC-Aro media, suggesting the presence of at least one additional isozyme. To confirm this result, a 222-bp DNA fragment was amplified by the polymerase chain reaction (PCR) from genomic DNA prepared from the homozygous aro3-deletion mutant, using a degenerate primer based on a conserved N-terminal region of Aro3p plus a degenerate comeback primer encoding a conserved region of the protein that lies within the deleted portion of the gene. The nucleotide sequence of this PCR fragment predicts a 74-amino acid DAHP synthase-related protein which shows strong homology to Aro3p from S. cerevisiae and C. albicans, but even greater homology (78% identity) to S. cerevisiae Aro4p. We conclude that cells of C. albicans contain a second Aro4p-related DAHP synthase. |
| Databáze: | OpenAIRE |
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