Hybrid wound dressings with controlled release of antibiotics: Structure-release profile effects and in vivo study in a guinea pig burn model
| Autor: | Yehuda Ullmann, Maoz Shemesh, Aviad Keren, Amos Gilhar, Maya Baranes-Zeevi, Nyra Goldstein, Eytan Mazor, Dana Egozi, Meital Zilberman, Israela Berdicevsky |
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| Rok vydání: | 2015 |
| Předmět: |
Antibiotic drug
Materials science medicine.drug_class Guinea Pigs Antibiotics Biomedical Engineering Biocompatible Materials Biochemistry Biomaterials Guinea pig In vivo medicine Animals Molecular Biology Skin Wound Healing integumentary system General Medicine Bandages Controlled release Anti-Bacterial Agents Disease Models Animal Delayed-Action Preparations Female Gentamicin Gentamicins Burns Wound healing Bandage Biotechnology Biomedical engineering medicine.drug |
| Zdroj: | Acta Biomaterialia. 22:155-163 |
| ISSN: | 1742-7061 |
| DOI: | 10.1016/j.actbio.2015.04.029 |
| Popis: | Over the last decades, wound dressings have evolved from a crude traditional gauze dressing to tissue-engineered scaffolds. Many types of wound dressing formats are commercially available or have been investigated. We developed and studied hybrid bilayer wound dressings which combine a drug-loaded porous poly(dl-lactic-co-glycolic acid) top layer with a spongy collagen sublayer. Such a structure is very promising because it combines the advantageous properties of both layers. The antibiotic drug gentamicin was incorporated into the top layer for preventing and/or defeating infections. In this study, we examined the effect of the top layer's structure on the gentamicin release profile and on the resulting in vivo wound healing. The latter was tested on a guinea pig burn model, compared to the neutral non-adherent dressing material Melolin® (Smith & Nephew) and Aquacel® Ag (ConvaTec). The release kinetics of gentamicin from the various studied formulations exhibited burst release values between 8% and 38%, followed by a drug elution rate that decreased with time and lasted for at least 7 weeks. The hybrid dressing, with relatively slow gentamicin release, enabled the highest degree of wound healing (28%), which is at least double that obtained by the other dressing formats (8-12%). It resulted in the lowest degree of wound contraction and a relatively low amount of inflammatory cells compared to the controls. This dressing was found to be superior to hybrid wound dressings with fast gentamicin release and to the neat hybrid dressing without drug release. Since this dressing exhibited promising results and does not require frequent bandage changes, it offers a potentially valuable concept for treating large infected burns. |
| Databáze: | OpenAIRE |
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