Expression profiling of budding cells in colorectal cancer reveals an EMT-like phenotype and molecular subtype switching
| Autor: | Linde De Smedt, Gert De Hertogh, Hans Prenen, Jeroen Dekervel, Dominiek Smeets, Thomas Tousseyn, David Barras, Diether Lambrechts, Jasper Wouters, Eva Galle, Sabine Tejpar, Olivier Govaere, Sofie Palmans, Daan Andel, Madeleine Suffiotti, Bram Boeckx, Xavier Sagaert |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Cancer Research Pathology medicine.medical_specialty Epithelial-Mesenchymal Transition Cell division colorectal cancer budding epithelial–mesenchymal transition Transcriptome 03 medical and health sciences 0302 clinical medicine Gene expression Medicine Humans Neoplasm Invasiveness Molecular Diagnostics Aged Cell Proliferation Aged 80 and over Budding business.industry Gene Expression Profiling Margins of Excision Gene signature Middle Aged Phenotype Gene expression profiling 030104 developmental biology Oncology Tissue Array Analysis 030220 oncology & carcinogenesis Cancer cell Cancer research Female Human medicine business Colorectal Neoplasms Cell Division Genes Switch |
| Zdroj: | British Journal of Cancer The British journal of cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | Background: Tumour budding, described as the presence of single cells or small clusters of up to five tumour cells at the invasive margin, is established as a prognostic marker in colorectal carcinoma. In the present study, we aimed to investigate the molecular signature of tumour budding cells and the corresponding tumour bulk. Methods: Tumour bulk and budding areas were microdissected and processed for RNA-sequencing. As little RNA was obtained from budding cells, a special low-input mRNA library preparation protocol was used. Gene expression profiles of budding as compared with tumour bulk were investigated for established EMT signatures, consensus molecular subtype (CMS), gene set enrichment and pathway analysis. Results: A total of 296 genes were differentially expressed with an FDR |
| Databáze: | OpenAIRE |
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