Cloning and Characterization of a Tumor-Associated Antigen, β-Casein-like Protein
Autor: | Ikuhito Yamanaka, Takahiro Suzuki, Tsuyoshi Baba, Ryuichi Kudo, Motoiki Koizumi, Satoko Yamashita |
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Rok vydání: | 2001 |
Předmět: |
DNA
Complementary medicine.drug_class Molecular Sequence Data Biophysics Down-Regulation Uterine Cervical Neoplasms Adenocarcinoma Biology Molecular cloning Transfection Monoclonal antibody Cell morphology Biochemistry Antigen Antigens Neoplasm Complementary DNA Cell Adhesion Tumor Cells Cultured medicine Humans Amino Acid Sequence RNA Messenger Northern blot Cloning Molecular Molecular Biology Cell Size Cloning Base Sequence Antibodies Monoclonal Caseins Cell Biology Fibroblasts Blotting Northern Molecular biology Uterine Neoplasms Female Cell Division |
Zdroj: | Biochemical and Biophysical Research Communications. 284:340-345 |
ISSN: | 0006-291X |
Popis: | Monoclonal antibody (MAb) 1C5 reacts with 87% of uterine cervical adenocarcinoma and bovine β -casein, but not with squamous cell carcinoma. To clarify the characteristics of the antigen (β-casein-like protein; BCLP) recognized by MAb 1C5, molecular cloning was performed using 5′ rapid amplification of the cDNA ends (5′ RACE) and the oligo-capping method. The protein predicted from the cDNA consisting of 937 nucleotides comprises 222 amino acids. The BCLP gene and deduced amino acid sequences were novel and showed no similarity to known cancer-associated genes in the database. Northern blot analysis showed that a 1.1 kb transcript was ubiquitously expressed in cancer cell lines and was predominantly expressed in uterine cervical adenocarcinoma. To clarify the function of BCLP, BCLP cDNA was transfected into L929 cells, resulting in a significant increase in cell area, a downregulation of cell growth rate and a decrease in cell attachment. We conclude that BCLP might be associated with cell morphology and a regulation of growth pattern of tumor. |
Databáze: | OpenAIRE |
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