A Dense Linkage Map of Lake Victoria Cichlids Improved the Pundamilia Genome Assembly and Revealed a Major QTL for Sex-Determination
| Autor: | Julia Schwarzer, Ole Seehausen, Joana I. Meier, Marcel P. Haesler, Philine G. D. Feulner |
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| Přispěvatelé: | Feulner, Philine GD [0000-0002-8078-1788], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Genetic Markers Genotype Genetic Linkage Quantitative Trait Loci sex determination Sequence assembly Quantitative trait locus QH426-470 Genome Evolution Molecular 03 medical and health sciences Genetic linkage Databases Genetic Genetics Animals sex chromosome evolution Gene Molecular Biology Genetics (clinical) Synteny Comparative genomics recombination rate Recombination Genetic biology XY system Pundamilia synteny RAD Chromosome Mapping High-Throughput Nucleotide Sequencing Cichlids Genomics Cichlidae Sex Determination Processes biology.organism_classification Genetics of Sex Lakes 030104 developmental biology Evolutionary biology Pundamilia pundamilia amh 570 Life sciences |
| Zdroj: | G3: Genes, Genomes, Genetics, Vol 8, Iss 7, Pp 2411-2420 (2018) G3: Genes|Genomes|Genetics Feulner, Philine; Schwarzer, Julia; Häsler, Marcel; Meier, Joana; Seehausen, Ole (2018). A Dense Linkage Map of Lake Victoria Cichlids Improved the Pundamilia Genome Assembly and Revealed a Major QTL for Sex-Determination. G3 Genes Genomes Genetics, 8(7), pp. 2411-2420. Genetics Society of America 10.1534/g3.118.200207 |
| DOI: | 10.7892/boris.116922 |
| Popis: | Genetic linkage maps are essential for comparative genomics, high quality genome sequence assembly and fine scale quantitative trait locus (QTL) mapping. In the present study we identified and genotyped markers via restriction-site associated DNA (RAD) sequencing and constructed a genetic linkage map based on 1,597 SNP markers of an interspecific F2 cross of two closely related Lake Victoria cichlids (Pundamilia pundamilia and P. sp. “red head”). The SNP markers were distributed on 22 linkage groups and the total map size was 1,594 cM with an average marker distance of 1.01 cM. This high-resolution genetic linkage map was used to anchor the scaffolds of the Pundamilia genome and estimate recombination rates along the genome. Via QTL mapping we identified a major QTL for sex in a ∼1.9 Mb region on Pun-LG10, which is homologous to Oreochromis niloticus LG 23 (Ore-LG23) and includes a well-known vertebrate sex-determination gene (amh). |
| Databáze: | OpenAIRE |
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