Identification and functional analysis ofSalmonella entericaserovar Typhimurium PmrA-regulated genes
| Autor: | Angela M. Prouty, Rita Tamayo, John S. Gunn |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Salmonella typhimurium
Microbiology (medical) Salmonella DNA Complementary Consensus site Immunology Antimicrobial peptides Virulence medicine.disease_cause Regulon Microbiology Bacterial Proteins Complementary DNA Drug Resistance Bacterial medicine Immunology and Allergy Polymyxins RNA Messenger Promoter Regions Genetic Gene Oligonucleotide Array Sequence Analysis biology Gene Expression Profiling Gene Expression Regulation Bacterial General Medicine biology.organism_classification Adaptation Physiological Arabinose RNA Bacterial Lipid A Infectious Diseases Genes Bacterial Salmonella enterica Mutation Antimicrobial Cationic Peptides Protein Binding |
| Zdroj: | FEMS Immunology & Medical Microbiology. 43:249-258 |
| ISSN: | 1574-695X 0928-8244 |
| DOI: | 10.1016/j.femsim.2004.08.007 |
| Popis: | The PmrA-PmrB two-component regulatory system of Salmonella enterica serovar Typhimurium is activated in vivo and plays an important role in resistance to cationic antimicrobial peptides. Resistance is partly mediated by modifications to the lipopolysaccharide. To identify new PmrA-regulated genes, microarray analysis was undertaken comparing cDNA derived from PmrA-constitutive and PmrA-null strains. A combination of RT-PCR and transcriptional analysis confirmed the inclusion of six new loci in the PmrA-PmrB regulon: STM1253, STM1269, STM4118, STM0459, STM3968 and STM4568. These loci did not affect the ability to grow in high iron conditions, the ability to modify lipid A with aminoarabinose, or virulence. STM4118, a putative phosphoethanolamine phosphotransferase, had a minor effect on polymyxin resistance, whereas the remaining genes had no role in polymyxin resistance. Although several of the identified loci lacked the consensus PmrA binding site, PmrA was demonstrated to bind the promoter of a PmrA-activated gene lacking the consensus site. A more complete definition of the PmrA-PmrB regulon will provide a better understanding of its role in host and non-host environments. |
| Databáze: | OpenAIRE |
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