Stonin1 mediates endocytosis of the proteoglycan NG2 and regulates focal adhesion dynamics and cell motility
| Autor: | Sonia Waiczies, Eberhard Krause, Philipp Trnka, Tanja Maritzen, William B. Stallcup, Rainer Glass, Thoralf Niendorf, Fabian Feutlinske, Min-Chi Ku, Marietta Browarski |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Endocytic cycle
Integrin General Physics and Astronomy Motility Fluorescent Antibody Technique Biology Endocytosis General Biochemistry Genetics and Molecular Biology Article Focal adhesion Mice Cell surface receptor Cell Movement Tandem Mass Spectrometry Animals Humans Antigens Receptor Mice Knockout Focal Adhesions Multidisciplinary Photobleaching Membrane Proteins Cell migration General Chemistry Fibroblasts Flow Cytometry Cell biology Adaptor Proteins Vesicular Transport HEK293 Cells Cardiovascular and Metabolic Diseases biology.protein Proteoglycans Transcription Factors General Chromatography Liquid |
| Zdroj: | Nature Communications Nature Communications, 6: 8535 |
| ISSN: | 2041-1723 |
| Popis: | Cellular functions, ranging from focal adhesion (FA) dynamics and cell motility to tumour growth, are orchestrated by signals cells receive from outside via cell surface receptors. Signalling is fine-tuned by the exo–endocytic cycling of these receptors to control cellular responses such as FA dynamics, which determine cell motility. How precisely endocytosis regulates turnover of the various cell surface receptors remains unclear. Here we identify Stonin1, an endocytic adaptor of unknown function, as a regulator of FA dynamics and cell motility, and demonstrate that it facilitates the internalization of the oncogenic proteoglycan NG2, a co-receptor of integrins and platelet-derived growth factor receptor. Embryonic fibroblasts obtained from Stonin1-deficient mice display a marked surface accumulation of NG2, increased cellular signalling and defective FA disassembly as well as altered cellular motility. These data establish Stonin1 as a specific adaptor for the endocytosis of NG2 and as an important factor for FA dynamics and cell migration. Signalling is often fine-tuned by the exo-endocytic cycling of cell surface receptors. Here, the authors show that the endocytic adaptor protein Stonin1 is important for the endocytosis of NG2, a co-receptor for extracellular matrix and growth factors, and that loss of Stonin1 alters cell motility. |
| Databáze: | OpenAIRE |
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