Dictyostelium discoideum gene family contains a long internal amino acid repeat
| Autor: | Roberto Giorda, David R. Shaw, Herbert L. Ennis, John E. Blume, Tetsuo Ohmachi |
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| Rok vydání: | 1991 |
| Předmět: |
Genes
Fungal Molecular Sequence Data Restriction Mapping Dictyostelium discoideum Homology (biology) Fungal Proteins Gene Expression Regulation Fungal Complementary DNA Genetics Spore germination Gene family Dictyostelium Amino Acid Sequence RNA Messenger Gene Repetitive Sequences Nucleic Acid chemistry.chemical_classification biology RNA Fungal Cell Biology Spores Fungal Blotting Northern biology.organism_classification Amino acid Blotting Southern Kinetics chemistry Multigene Family Developmental Biology |
| Zdroj: | Developmental Genetics. 12:133-138 |
| ISSN: | 1520-6408 0192-253X |
| DOI: | 10.1002/dvg.1020120121 |
| Popis: | Two different cDNA clones denoted pT0270-6 and pT0270-11 represent two mRNAs that are developmentally regulated during spore germination in Dictyosteliurn discoideum. The respective mRNAs are found only during early germination and are not present in other stages of growth or multicellular development. Four different genomic clones that hybridize to sequences that are common to both of the 270 cDNA clones were isolated from Dictyostelium libraries and se- quenced. Two are the genes for the two cDNAs, and the other two represent genes that do not seem to be transcribed. All four genomic se- quences possess a very unusual internal feature in the deduced protein sequences composed of a monotonous repeat of the tetrapeptide threonine- glutamic acid-threonine-proline. The other portions of the proteins have no homology among them- selves. The deduced protein corresponding to the 270-6 gene is very similar to avocado (Persea arnericana) cellulase. Since cellulose in the spore wall has to be digested during spore germination this suggests that this protein may function as an endo-( 1,4)-beta-D-glucanase during germination. |
| Databáze: | OpenAIRE |
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