Sex difference in the antidiuretic activity of vasopressin in the rat
| Autor: | Leonard Share, David P. Brooks, Mark Pullen, E. J. Stack, Joan T. Crofton, Yi-Xin Wang, Richard M. Edwards, Ponnal Nambi |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
Receptors Vasopressin medicine.medical_specialty Vasopressin Vasopressins Physiology Biology Rats Sprague-Dawley Excretion Body Water Physiology (medical) Internal medicine Cyclic AMP medicine Animals Kidney Tubules Collecting Receptor Kidney Medulla Sex Characteristics Kidney Adenosine Diuresis Rats Arginine Vasopressin Free water clearance Dissociation constant Endocrinology medicine.anatomical_structure Urine osmolality Female medicine.drug |
| Zdroj: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 265:R1284-R1290 |
| ISSN: | 1522-1490 0363-6119 |
| DOI: | 10.1152/ajpregu.1993.265.6.r1284 |
| Popis: | A possible gender difference in the antidiuretic activity of vasopressin was studied in male and female Sprague-Dawley rats. Infusion of vasopressin (3-100 pg.kg-1.min) into conscious, chronically instrumented water-loaded rats resulted in a dose-dependent antidiuresis in both male and female rats. Male rats, however, were more than three times more sensitive to vasopressin than female rats. Thus the effective doses of vasopressin (pg.kg-1.min-1) to decrease urine flow to 30 microliters.min-1.100 g-1 (18 +/- 5 in males; 58 +/- 12 in females), to increase urine osmolality to 600 mosmol/kgH2O (35 +/- 5 in males; 119 +/- 15 in females), and to decrease free water clearance to 30 microliters.min-1.100 g-1 (8 +/- 3 in males; 28 +/- 7 in females) were significantly (P < 0.05) lower in males. Furthermore, in vitro studies in papillary collecting duct cells demonstrated a significantly higher density of vasopressin V2 receptors and a greater ability of vasopressin to stimulate adenosine 3',5'-cyclic monophosphate (cAMP) accumulation in males than in females. Vasopressin V2-receptor density (maximum binding) was 359 +/- 47 and 238 +/- 22 fmol/mg in male and female rats, respectively (P < 0.05). There was no difference in apparent dissociation constants (Kd). Vasopressin resulted in a dose-dependent increase in cAMP accumulation in papillary collecting duct cells, and at the highest concentration of vasopressin used (10(-8) M) cAMP increased from 44 +/- 10 to 182 +/- 51 fmol/micrograms protein in males and from 30 +/- 4 to 91 +/- 18 fmol/micrograms protein in females (P < 0.05). (ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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