Cutting Edge: Priming of NK Cells by IL-18
| Autor: | Nicolas Fuseri, Thierry Walzer, Eric Vivier, Bruce Beutler, Lena Alexopoulou, Laurent Brossay, Marlowe S. Tessmer, Bernhard Ryffel, Marc Dalod, Julie Chaix, Kasper Hoebe |
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| Přispěvatelé: | Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Department of Molecular Microbiology and Immunology, Brown University, Division of Molecular Immunology, University of Cincinnati (UC)-Cincinnati Children's Hospital Medical Center, Immunologie et Embryologie Moléculaires (IEM), Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS), Department of Immunology and Microbial Science, Scripps Research Institute, The Scripps Research Institute [La Jolla, San Diego] |
| Rok vydání: | 2008 |
| Předmět: |
MESH: Signal Transduction
MESH: Interleukin-12 MESH: Killer Cells Natural MESH: Cross-Priming MESH: Interferon-gamma Immunology Priming (immunology) Stimulation Biology MESH: Mice Knockout Article Interferon-gamma Mice 03 medical and health sciences Cross-Priming 0302 clinical medicine MESH: Mice Inbred C57BL In vivo Animals Immunology and Allergy MESH: Animals Secretion MESH: Mice Cells Cultured 030304 developmental biology Mice Knockout 0303 health sciences Messenger RNA Effector Interleukin-18 Interleukin-12 Cell biology Killer Cells Natural Mice Inbred C57BL Myeloid Differentiation Factor 88 [SDV.IMM]Life Sciences [q-bio]/Immunology Interleukin 18 MESH: Interleukin-18 Ex vivo MESH: Cells Cultured MESH: Myeloid Differentiation Factor 88 Signal Transduction 030215 immunology |
| Zdroj: | Journal of Immunology Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2008, 181 (3), pp.1627-31 Scopus-Elsevier ResearcherID Journal of Immunology, 2008, 181 (3), pp.1627-31 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.181.3.1627 |
| Popis: | Recent evidence suggests that NK cells require priming to display full effector activity. In this study, we demonstrate that IL-18 contributed to this phenomenon. IL-18 signaling-deficient NK cells were found to be unable to secrete IFN-γ in response to ex vivo stimulation with IL-12. This was not due to a costimulatory role of IL-18, because blocking IL-18 signaling during the ex vivo stimulation with IL-12 did not alter IFN-γ production by wild-type NK cells. Rather, we demonstrate that IL-18 primes NK cells in vivo to produce IFN-γ upon subsequent stimulation with IL-12. Importantly, IL-12-induced IFN-γ transcription by NK cells was comparable in IL-18 signaling-deficient and -sufficient NK cells. This suggests that priming by IL-18 leads to an improved translation of IFN-γ mRNA. These results reveal a novel type of cooperation between IL-12 and IL-18 that requires the sequential action of these cytokines. |
| Databáze: | OpenAIRE |
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