Molecular analysis of the Escherichia coli recO gene
| Autor: | Susan T. Lovett, L E Gilson, Richard D. Kolodner, Paul T. Morrison |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Restriction Mapping Biology Microbiology Insertional mutagenesis Structure-Activity Relationship Start codon Bacterial Proteins Escherichia coli Deletion mapping Amino Acid Sequence Molecular Biology Peptide sequence Southern blot Genetics Base Sequence Genetic Complementation Test Nucleic acid sequence Molecular biology Open reading frame Genes Bacterial Codon usage bias DNA Transposable Elements Plasmids Research Article |
| Zdroj: | Journal of bacteriology. 171(7) |
| ISSN: | 0021-9193 |
| Popis: | The plasmid pLC7-47, which contains lep, rnc, and era, was found to complement the UV-sensitive and recombination-deficient phenotypes caused by the recO1504::Tn5 mutation. Southern blotting analysis demonstrated that pLC7-47 contained a segment of Escherichia coli DNA that covered the region of the E. coli chromosome containing the recO1504::Tn5 mutation. A combination of deletion mapping and insertional mutagenesis localized the recO-complementing region to an approximately 1-kilobase region of a 1.6-kilobase BamHI fragment. The DNA sequence of the 1.6-kilobase BamHI fragment was determined and contained part of era and a 726-base-pair recO open reading frame. The recO open reading frame contained three possible translation start codons and could potentially encode a polypeptide of Mr 26,000. Computer analysis indicated that the putative RecO protein had suboptimal codon usage and did not show significant homology with previously identified proteins whose sequences were present in protein data bases. A combination of primary sequence analysis and secondary structure predictions suggested that recO contains a mononucleotide-binding fold. |
| Databáze: | OpenAIRE |
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