Telomerase activity in ductal carcinoma in situ of the breast
| Autor: | Y Bomstein, S Liverant, T Zehavi, Ruth Zemer, Ehud Klein, Y Shapira, Ami Klein, J Bernehim, Z Kaufman, B. Shpitz, Shulamit Zimlichman |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Cancer Research Pathology medicine.medical_specialty Telomerase Mammary gland Breast Neoplasms Biology medicine.disease_cause Polymerase Chain Reaction Breast cancer Biopsy medicine Humans Neoplasm Invasiveness Breast skin and connective tissue diseases neoplasms Aged medicine.diagnostic_test Carcinoma in situ Carcinoma Ductal Breast Ductal carcinoma Middle Aged medicine.disease Immunohistochemistry body regions medicine.anatomical_structure Carcinoma Intraductal Noninfiltrating Oncology Female Carcinogenesis |
| Zdroj: | Breast cancer research and treatment. 58(1) |
| ISSN: | 0167-6806 |
| Popis: | Telomerase plays an important role in maintaining the stability of the chromosomes. Activity of telomerase has been detected in proliferating and immortalized cell lines and in a number of malignant tumors including invasive breast cancer. The aim of the study was to examine telomerase activity in ductal carcinoma in situ (DCIS), which is considered to be a precursor lesion of infiltrating breast carcinoma, using a PCR-based telomerase activity protocol (TRAP). We examined 35 samples obtained from histologically confirmed breast biopsies, including 13 normal breast tissues, 11 infiltrating ductal carcinoma (IDC), nine DCIS, and two DCIS with microinvasion. Telomerase activity was demonstrated in 8/9 samples of DCIS, both samples of DCIS with microinvasion, and all but one sample of IDC. Normal breast tissue had no demonstrable telomerase activity. Our results indicate that telomerase is activated frequently in early breast carcinogenesis, although its utilization as a biomarker in DCIS is questionable. |
| Databáze: | OpenAIRE |
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