Regulation of calcium fluxes by GPX8, a type-II transmembrane peroxidase enriched at the mitochondria-associated endoplasmic reticulum membrane

Autor: Alessandro Rimessi, Tiziana Anelli, Paolo Pinton, Edgar Djaha Yoboue, Roberto Sitia
Přispěvatelé: Yoboue, Edgar Djaha, Rimessi, Alessandro, Anelli, Tiziana, Pinton, Paolo, Sitia, Roberto
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Physiology
Clinical Biochemistry
Socio-culturale
Biology
Biochemistry
03 medical and health sciences
Cytosol
mitochondria associated membrane (MAM)
membrane topology
Protein Domains
Calcium flux
Humans
glutathione peroxidase
Molecular Biology
General Environmental Science
Endoplasmic reticulum membrane
030102 biochemistry & molecular biology
Endoplasmic reticulum
glutathione peroxidases
Cell Membrane
STIM1
Cell Biology
calcium (Ca2+) signaling
endoplasmic reticulum
mitochondria associated membrane (MAM)
calcium (Ca2+) signaling
glutathione peroxidases
membrane topology

Transmembrane protein
Cell biology
Mitochondria
stomatognathic diseases
Transmembrane domain
endoplasmic reticulum
030104 developmental biology
Peroxidases
Membrane topology
General Earth and Planetary Sciences
Calcium
human activities
HeLa Cells
Popis: Glutathione peroxidases (GPXs) are enzymes that are present in almost all organisms with the primary function of limiting peroxide accumulation. In mammals, two of the eight members (GPX7 and GPX8) reside in the endoplasmic reticulum (ER). A peculiar feature of GPX8 is the concomitant presence of a conserved N-terminal transmembrane domain (TMD) and a C-terminal KDEL-like motif for ER localization. Aims: Investigating whether and how GPX8 impacts Ca2+ homeostasis and signaling. Results: We show that GPX8 is enriched in mitochondria-associated membranes and regulates Ca2+ storage and fluxes. Its levels correlate with [Ca2+]ER, and cytosolic and mitochondrial Ca2+ fluxes. GPX7, which lacks a TMD, does not share these properties. Deleting or replacing the GPX8 TMD with an unrelated N-terminal membrane integration sequence abolishes all effects on Ca2+ fluxes, whereas appending the GPX8 TMD to GPX7 transfers the Ca2+-regulating properties. Innovation and Conclusion: The notion that the TMD of GPX8, i...
Databáze: OpenAIRE
Pro tento záznam nejsou dostupné žádné jednotky.