Autografting of bone marrow mesenchymal stem cells alleviates streptozotocin-induced diabetes in miniature pigs: Real-time tracing with MRI in vivo
| Autor: | Daizhi Yang, Yu Wang, Hua Liang, Bao-Ying Li, Jinhua Yan, De-Dong Ma, Da-Yue Liu, Xiaoyan Xiao, Yunfeng Shen, Kuan-Xiao Tang, Ying-Ming Chen, Jianping Weng, Fei Yu |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Blood Glucose
Male diabetes mellitus type 1 medicine.medical_specialty endocrine system diseases Miniature pig Swine medicine.medical_treatment Sus scrofa miniature pigs Mesenchymal Stem Cell Transplantation Transplantation Autologous Diabetes Mellitus Experimental autologous transplantation Diabetes mellitus Internal medicine Genetics medicine Animals Insulin Glucose homeostasis Type 1 diabetes Glucose tolerance test C-Peptide biology medicine.diagnostic_test bone marrow mesenchymal stem cells Mesenchymal Stem Cells Articles General Medicine Glucose Tolerance Test cell therapeutics biology.organism_classification medicine.disease Streptozotocin Magnetic Resonance Imaging Transplantation Endocrinology Swine Miniature medicine.drug |
| Zdroj: | International Journal of Molecular Medicine |
| ISSN: | 1791-244X 1107-3756 |
| DOI: | 10.3892/ijmm.2014.1729 |
| Popis: | Cellular replacement therapy for diabetes mellitus (DM) has received much attention. In this study, we investigated the effect of transplantation of autologous bone marrow-derived mesenchymal stem cells (ABMSCs) in streptozotocin (STZ)-induced diabetic miniature pigs. Miniature pig BMSCs were cultured, labeled with superparamagnetic iron oxide (SPIO) and transplanted into the pancreas of diabetic miniature pigs through targeted intervention. Blood glucose levels, intravenous and oral glucose tolerance test (OGTT), serum insulin, C-peptide and islets histology were analyzed. These transplanted cells were then identified by magnetic resonance imaging (MRI). The results showed that transplantation of ABMSCs reversed STZ-induced diabetes in miniature pigs. Blood glucose levels, intravenous, OGTT, serum insulin and C-peptide were significantly recovered in the diabetic minipigs with the autologous BMSC (DMAB) transplantation group. In addition, the number of islets was significantly increased in this group compared to the diabetic minipig control (DMC) group with conventional therapy. These data suggested the implantation of autologous BMSCs for type 1 diabetes treatment can partially restore the function of islet β cells and maintain blood glucose homeostasis. Transplanted autologous BMSCs may improve islet repairing by differentiating for new islets and change pancreatic microcirculation and be identified in a real-time manner using MRI in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|