Characterization, molecular cloning and localization of calreticulin in Eisenia fetida earthworms
| Autor: | Marcela Šilerová, Ellen Kauschke, Petra Procházková, Martin Bilej, Radka Josková, Ludmila Tučková |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Cell signaling Eisenia fetida DNA Complementary Immunocytochemistry Molecular Sequence Data In situ hybridization Genetics Animals Tissue Distribution Amino Acid Sequence Cloning Molecular Oligochaeta Peptide sequence In Situ Hybridization Phylogeny biology Base Sequence Endoplasmic reticulum General Medicine biology.organism_classification Molecular biology Immunohistochemistry Recombinant Proteins Ventral nerve cord biology.protein Female Calreticulin |
| Zdroj: | Gene. 397(1-2) |
| ISSN: | 0378-1119 |
| Popis: | Calreticulin is a highly conserved calcium-binding protein affecting many cellular processes inside and outside of the endoplasmic reticulum (ER). It participates in the regulation of Ca2+ homeostasis, acts as a chaperone and modulates gene transcription, integrin-mediated cell signalling as well as cell adhesion. Here we report on the sequence characterization of a calreticulin-coding cDNA of Eisenia fetida earthworms. The neighbor-joining phylogeny tree constructed based on the deduced amino acid sequence indicates a common origin of the E. fetida calreticulin molecule and that of mollusks. A polyclonal anti-calreticulin antibody used for immunocytochemistry and immunohistochemistry localized the protein in the mesenchymal lining of the coelomic cavity and in coelomocytes of E. fetida. In situ hybridization revealed high expression of E. fetida calreticulin in various cells and tissues, namely epidermis, neurons of the ventral nerve cord, intestine, sperms, body wall muscles and some coelomocytes. Real-time PCR confirmed the strong expression of calreticulin in the nervous system, particularly in cerebral ganglia, in body wall muscles and in seminal vesicles. Moreover, a high calreticulin expression was measured in the muscular pharynx. |
| Databáze: | OpenAIRE |
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