Mapping of polyomavirus DNA replicative intermediates by two-dimensional gel analysis using chemiluminescent detection
| Autor: | Luis P. Villarreal, Zhiyong You |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
DNA synthesis
DNA replication Substrate (chemistry) Biology Sensitivity and Specificity Combinatorial chemistry Molecular biology Cell Line law.invention Mice chemistry.chemical_compound chemistry Cell culture law Virology DNA Viral Luminescent Measurements Animals Digoxigenin Electrophoresis Gel Two-Dimensional Polyomavirus Molecular probe DNA Chemiluminescence |
| Zdroj: | Journal of Virological Methods. 56:173-178 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/0166-0934(95)01950-2 |
| Popis: | Py DNA replicative intermediates (RIs) were mapped using a neutral/neutral two-dimensional (N/N 2D) technique by both chemiluminescent and radioactive detection. The nonradioactive method provides similar sensitivity to radioactive methods while possessing the advantages of stability of labeled probes, faster processing, multiple exposures, easy disposal and safety associated with nonradioactive detection. Application of this method to 2D gel analysis requires some technical modification to reduce background and save the substrate. The patterns of Py RIs with Afl II digestion suggest that bidirectional replication occurs during Py DNA synthesis in cell culture, but other unexpected structures of DNA replication are also seen. |
| Databáze: | OpenAIRE |
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