The critical role of cyclin D2 in adult neurogenesis
| Autor: | Grzegorz M. Wilczynski, Maria A. Ciemerych, Jacek Jaworski, Robert K. Filipkowski, Marcin Rylski, Anna Kowalczyk, Leszek Kaczmarek, Filip A. Konopacki, Piotr Sicinski |
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| Rok vydání: | 2004 |
| Předmět: |
Time Factors
Cyclin D Biology Hippocampus Mice Cyclin D1 Cyclin D2 Cyclins Report Animals RNA Messenger In Situ Hybridization Research Articles Cell Proliferation Cyclin Mice Knockout Neurons Mice Inbred BALB C Microscopy Confocal Reverse Transcriptase Polymerase Chain Reaction Dentate gyrus Neurogenesis Brain Cell Biology Immunohistochemistry Molecular biology Olfactory bulb Cell biology Mice Inbred C57BL Phenotype Bromodeoxyuridine nervous system biology.protein RNA Neural development |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Adult neurogenesis (i.e., proliferation and differentiation of neuronal precursors in the adult brain) is responsible for adding new neurons in the dentate gyrus of the hippocampus and in the olfactory bulb. We describe herein that adult mice mutated in the cell cycle regulatory gene Ccnd2, encoding cyclin D2, lack newly born neurons in both of these brain structures. In contrast, genetic ablation of cyclin D1 does not affect adult neurogenesis. Furthermore, we show that cyclin D2 is the only D-type cyclin (out of D1, D2, and D3) expressed in dividing cells derived from neuronal precursors present in the adult hippocampus. In contrast, all three cyclin D mRNAs are present in the cultures derived from 5-day-old hippocampi, when developmental neurogenesis in the dentate gyrus takes place. Thus, our results reveal the existence of molecular mechanisms discriminating adult versus developmental neurogeneses. |
| Databáze: | OpenAIRE |
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