Amoebae can promote the survival of Francisella species in the aquatic environment
| Autor: | Thomas Henry, Masa Knezevic, Max Maurin, Marina Šantić, Camille D. Brunet, Aurélie Hennebique, Sandrine Boisset, Amandine Martin, Julien Peyroux |
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| Přispěvatelé: | Centre Hospitalier Universitaire [Grenoble] (CHU), Translational microbial Evolution and Engineering (TIMC-TrEE), Translational Innovation in Medicine and Complexity / Recherche Translationnelle et Innovation en Médecine et Complexité - UMR 5525 (TIMC ), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
amoebae Epidemiology [SDV]Life Sciences [q-bio] 030106 microbiology Immunology Fresh Water Select agent microbial ecology Microbiology complex mixtures Tularemia 03 medical and health sciences Microbial ecology Virology Drug Discovery parasitic diseases medicine Francisella species Francisella Amoeba Francisella tularensis Microbial Viability biology Zoonosis General Medicine biochemical phenomena metabolism and nutrition medicine.disease biology.organism_classification bacterial infections and mycoses tularemia 030104 developmental biology Infectious Diseases Aquatic environment bacteria Parasitology Research Article |
| Zdroj: | Emerging microbes & infections Emerging microbes & infections, Earliest : Springer-Nature ; Latest : Taylor & Francis, 2021, ⟨10.1080/22221751.2021.1885999⟩ Emerging Microbes & Infections article-version (VoR) Version of Record |
| ISSN: | 2222-1751 |
| DOI: | 10.1080/22221751.2021.1885999⟩ |
| Popis: | International audience; Francisella tularensis, a tier select agent, is the causative bacterium of tularemia, a zoonosis with a large animal reservoir. However, F. tularensis, like many other Francisella species (including F. novicida and F. philomiragia), is assumed to have an aquatic reservoir. The mechanisms of Francisella species persistence in surface water remain poorly characterized. In this study, we deeply investigated the long-term interactions of the tularemia agent F. tularensis subsp. holarctica, F. novicida or F. philomiragia with amoebae of the Acanthamoeba species. In amoeba plate screening tests, all the Francisella species tested resisted the attack by amoebae. In in vitro infection models, intra-amoebic growth of Francisella varied according to the involved bacterial species and strains, but also the amoeba culture medium used. In co-culture models, the amoebae favoured Francisella survival over 16 days. Such enhanced survival was likely dependent on direct contact between bacteria and amoebae for F. novicida and on amoeba-excreted compounds for F. novicida and for F. tularensis. In a spring water co-culture model, amoebae again enhanced F. novicida survival and preserved bacterial morphology. Overall, our results demonstrate that amoebae likely promote Francisella survival in aquatic environments, including the tularemia agent F. tularensis. However, bacteria-amoebae interactions are complex and depend on the Francisella species considered. |
| Databáze: | OpenAIRE |
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