Association of cytokine gene polymorphisms with osteoarthritis susceptibility
| Autor: | Florin Burada, Simona Sosoi, Emilian Stefan, Mihai Gabriel Cucu, Mihai Ioana, Otilia Constantina Rogoveanu, Anca Oana Docea, Daniela Calina, Emilia Burada |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research education.field_of_study business.industry medicine.medical_treatment Population Single-nucleotide polymorphism Articles General Medicine Pathogenesis 03 medical and health sciences 030104 developmental biology Cytokine Immunology and Microbiology (miscellaneous) Genotype Immunology medicine SNP Allele education business Genotyping |
| Zdroj: | Experimental and Therapeutic Medicine. |
| ISSN: | 1792-1015 1792-0981 |
| Popis: | Osteoarthritis (OA) is a multifactorial disease characterized by low-grade inflammatory processes that are mediated initially by the cells and factors of the innate immune system. In addition to their key role in inflammation, cytokines contribute to the pathogenesis of OA through angiogenesis and chemotaxis. The purpose of the present case-control study was to investigate a possible association of four cytokine single nucleotide polymorphisms (SNPs), IL-4R −3223C>T (rs2057768), IL-8 −251T>A (rs4073), IL-10 −1082A>G (rs1800896) and TNF -A-308G>A (rs1800629) with OA susceptibility. Genomic DNA was isolated from blood samples collected from 305 Romanian subjects (90 patients with OA and 215 controls) and the genotyping of the SNPs was performed by TaqMan allelic discrimination polymerase chain reaction using predesigned assays. Our data indicated a significant association for IL-4R rs2057768 C>T SNP. The subjects that carried the CT genotype were at a higher risk for OA (OR 2.03, 95% CI: 1.21–3.42, P=0.007) compared with those that had the CC genotype. Furthermore, the carriers of the T allele were at a 1.9 fold higher risk for OA (OR 1.92; 95% CI, 1.17–3.17; P=0.009) in a dominant model. The association remained significant only for knee OA in the subgroups analysis. No correlations were observed between the other remaining SNPs and OA. The results suggested that the IL-4R rs2057768 SNP could contribute to OA susceptibility in the Romanian population, providing novel evidence for the involvement of IL-4/IL-4R pair in the pathogenesis of OA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|