Molecular characterisation of Czech Clostridium difficile isolates collected in 2013-2015
| Autor: | Jana Matejkova, Otakar Nyc, Mark H. Wilcox, Franz Allerberger, Marcela Krutova, Ed J. Kuijper |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine Microbiology (medical) Adolescent Genotype Bacterial Toxins 030106 microbiology Capillary electrophoresis ribotyping Biology Molecular typing Ribotyping Microbiology Young Adult 03 medical and health sciences Humans Typing Child Aged Czech Republic tcdC Aged 80 and over Molecular Epidemiology Clostridioides difficile Nosocomial pathogens Infant Newborn Genetic Variation Infant General Medicine Clostridium difficile Middle Aged Sequence types Hospitals Housekeeping gene Infectious Diseases Child Preschool Clostridium Infections Multilocus sequence typing Female Toxin genes Multilocus Sequence Typing MLST |
| Zdroj: | International Journal of Medical Microbiology, 306(7), 479-485 |
| DOI: | 10.1016/j.ijmm.2016.07.003 |
| Popis: | Clostridium difficile is a leading nosocomial pathogen and molecular typing is a crucial part of monitoring its occurrence and spread. Over a three-year period (2013-2015), clinical C. difficile isolates from 32 Czech hospitals were collected for molecular characterisation. Of 2201 C. difficile isolates, 177 (8%) were non-toxigenic, 2024 (92%) were toxigenic (tcdA and tcdB) and of these, 677 (33.5%) carried genes for binary toxin production (cdtA, cdtB). Capillary-electrophoresis (CE) ribotyping of the 2201 isolates yielded 166 different CE-ribotyping profiles, of which 53 were represented by at least two isolates for each profile. Of these, 29 CE-ribotyping patterns were common to the Leeds-Leiden C. difficile reference strain library and the WEBRIBO database (83.7% isolates), and 24 patterns were recognized only by the WEBRIBO database (11.2% isolates). Isolates belonging to these 53 CE-ribotyping profiles comprised 94.9% of all isolates. The ten most frequent CE-ribotyping profiles were 176 (n=588, 26.7%), 001 (n=456, 20.7%), 014 (n=176, 8%), 012 (n=127, 5.8%), 017 (n=85, 3.9%), 020 (n=68, 3.1%), 596 (n=55, 2.5%), 002-like (n=45, 2.1%), 010 (n=35, 1.6%) and 078 (n=34, 1.6%). Multi-locus sequence typing (MLST) of seven housekeeping genes performed in one isolate of each of 53 different CE-ribotyping profiles revealed 40 different sequence types (STs). We conclude that molecular characterisation of Czech C. difficile isolates revealed a high diversity of CE-ribotyping profiles; the prevailing RTs were 001 (20.7%) and 176 (027-like, 26.7%). |
| Databáze: | OpenAIRE |
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