Alumina sol–gel/sonogel-carbon electrode based on acetylcholinesterase for detection of organophosphorus pesticides
| Autor: | Jean-Louis Marty, Hanane Zejli, Khalid R. Temsamani, Baohong Liu, Ignacio Naranjo-Rodriguez, José Luis Hidalgo-Hidalgo de Cisneros |
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| Rok vydání: | 2008 |
| Předmět: |
Detection limit
Chromatography technology industry and agriculture Reproducibility of Results macromolecular substances Polyethylene glycol Hydrogen-Ion Concentration Carbon Phase Transition Amperometry Analytical Chemistry Matrix (chemical analysis) chemistry.chemical_compound Organophosphorus Compounds Adsorption chemistry Nafion Acetylcholinesterase Aluminum Oxide Microscopy Electron Scanning Pesticides Electrodes Biosensor Ethylene glycol Nuclear chemistry |
| Zdroj: | Talanta. 77:217-221 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2008.06.010 |
| Popis: | Two new amperometric biosensors based on immobilization of acetylcholinesterase on a sonogel-carbon electrode for detection of organophosphorous compounds are proposed. The electrodes were prepared applying high-energy ultrasounds directly to the precursors. The first biosensor was obtained by simple entrapping acetylcholinesterase in Al 2 O 3 sol–gel matrix on the sonogel-carbon. The second biosensor was produced in a sandwich configuration. Its preparation involved adsorption of the enzyme and modification via a polymeric membrane such as polyethylene glycol and the ion-exchanger Nafion. The optimal enzyme loading was found to be 0.7 mIU. Both biosensors showed optimal activity in 0.2 M phosphate buffer, pH 7.0, at an operating potential of 210 mV. The detection limit achieved for chlorpyriphos-ethyl-oxon was 2.5 × 10 −10 M at a 10-min incubation time. |
| Databáze: | OpenAIRE |
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