Influence of Aptamer Surface Coverage on Small Target Recognition: A SPR and QCM-D Comparative Study
| Autor: | Nicolas Spinelli, Hannah MacDonald, Eric Defrancq, Hugues Bonnet, Liliane Coche-Guérente, Jérôme Dejeu, Angéline Van der Heyden |
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| Přispěvatelé: | Département de Chimie Moléculaire - Ingéniérie et Intéractions BioMoléculaires (DCM - I2BM), Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]) |
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Aptamer Nanotechnology 02 engineering and technology Quartz crystal microbalance Small target 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry General Energy Physical and Theoretical Chemistry Surface plasmon resonance 0210 nano-technology Biosensor |
| Zdroj: | Journal of Physical Chemistry C Journal of Physical Chemistry C, American Chemical Society, 2019, 123 (22), pp.13561-13568. ⟨10.1021/acs.jpcc.9b00845⟩ |
| ISSN: | 1932-7455 1932-7447 |
| Popis: | International audience; Aptamers have emerged as promising biorecognition elements for the development of biosensors. The present work focused on the direct detection, by surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring (QCM-D), of a low molecular weight (LMW) compound (less than 200 Da) with an aptamer receptor presented as an oriented monolayer on surface. These techniques are powerful for label-free, real-time characterization and quantification of molecular interactions at interfaces. Herein, we analyzed the influence of aptamer surface density on the recognition properties. A decrease of the surface |
| Databáze: | OpenAIRE |
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