Heat denatured enzymatically inactive recombinant chlamydial protease-like activity factor induces robust protective immunity against genital chlamydial challenge
| Autor: | Bernard P. Arulanandam, Weidang Li, M. Neal Guentzel, Guangming Zhong, Shankar J. Evani, James P. Chambers, Ashlesh K. Murthy, Bharat Kumar Reddy Chaganty |
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| Rok vydání: | 2009 |
| Předmět: |
Chlamydia muridarum
Protein Denaturation Hot Temperature medicine.medical_treatment Biology Article Microbiology Interferon-gamma Mice Immune system Immunity Endopeptidases medicine Animals Interferon gamma Lymphocytes Mice Inbred BALB C Vaccines Synthetic Protease General Veterinary General Immunology and Microbiology Public Health Environmental and Occupational Health Genitalia Female Chlamydia Infections biology.organism_classification Antibodies Bacterial Bacterial vaccine Vaccination Infectious Diseases Vaccines Inactivated Immunology Bacterial Vaccines Lymphogranuloma Venereum biology.protein Molecular Medicine Female Antibody Spleen medicine.drug |
| Zdroj: | Vaccine. 28(11) |
| ISSN: | 1873-2518 |
| Popis: | We have shown previously that vaccination with recombinant chlamydial protease-like activity factor (rCPAF) plus interleukin-12 as an adjuvant induces robust protective immunity against primary genital Chlamydia muridarum challenge in mice. Since CPAF is a protease, we compared the effects of enzymatically active and inactive (heat denatured) rCPAF to determine whether proteolytic activity is expendable for the induction of protective immunity against chlamydial challenge. Active, but not inactive, rCPAF immunization induced high levels of anti-active CPAF antibody, whereas both induced robust splenic CPAF-specific IFN-gamma production. Vaccination with active or inactive rCPAF induced enhanced vaginal chlamydial clearance as early as day 6 with complete resolution of infection by day 18, compared to day 30 in mock-vaccinated and challenged animals. Importantly, significant and comparable reductions in oviduct pathology were observed in active and inactive rCPAF-vaccinated mice compared to mock-vaccinated animals. Thus, rCPAF induced anti-chlamydial immunity is largely independent of enzymatic activity and secondary or higher order protein conformation. |
| Databáze: | OpenAIRE |
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