Downregulation of circulating miR-320a and target gene prediction in patients with diabetic retinopathy

Autor: Mirthz Lemos de Jesus, Marcelle SanJuan Ganem Prado, Lucilla Silva Oliveira Mendonça, Carla Martins Kaneto, Thaline Cunha de Goes
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
lcsh:Medicine
Down-Regulation
Bioinformatics
Real-Time Polymerase Chain Reaction
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Diabetes mellitus
microRNA
medicine
Cellular protein metabolic process
Humans
Circulating MicroRNA
lcsh:Science (General)
Gene
lcsh:QH301-705.5
Diabetic Retinopathy
business.industry
Gene Expression Profiling
lcsh:R
MicroRNA
General Medicine
Diabetic retinopathy
Biomarker
Middle Aged
medicine.disease
MicroRNAs
Research Note
030104 developmental biology
Gene Ontology
lcsh:Biology (General)
Gene Expression Regulation
030220 oncology & carcinogenesis
Circulating microRNAs
Biomarker (medicine)
Female
business
Cell aging
Biomarkers
lcsh:Q1-390
Signal Transduction
Zdroj: BMC Research Notes
BMC Research Notes, Vol 13, Iss 1, Pp 1-7 (2020)
ISSN: 1756-0500
Popis: Objective To evaluate the expression of a set of miRNAs to identify differentially expressed miRNAs that might be considered reliable biomarkers on Diabetic Retinopathy (DR) blood samples. Results Expression levels of MiR-320a, MiR-342-3p, MiR-155, MiR-99a, MiR-29a and MiR-27b were analyzed in 60 healthy controls, 48 Diabetes Melitus (DM) without DR patients and 62 DR patients by qRT-PCR. MiR-320a was shown to be downregulated in the plasma of DR patients compared with DM patients without DR and healthy subjects. Target genes were predicted using miRWalk3.0, miR targeting data and target gene interaction data were imported to Cytoscape to visualize and merge networks and top ranked predicted genes were run through Ontology Genes to perform enrichment analysis on gene sets and classification system to identify biological processes and reactome pathways associated with DR. Highly scored target genes of miR-320a were categorized for various biological processes, including negative regulation of cell aging, negative regulation of cellular protein metabolic process and regulation of cellular response to stress that are critical to the development of DR. Our findings suggest that MiR-320a may have a role in the pathogenesis of DR and may represent novel biomarkers for this disease.
Databáze: OpenAIRE
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