Neural stem cell isolation from the whole mouse brain using the novel FABP7-binding fluorescent dye, CDr3
| Autor: | Duanting Zhai, Young-Tae Chang, Seong-Wook Yun, Cheryl Leong, Beomsue Kim |
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| Rok vydání: | 2013 |
| Předmět: |
Boron Compounds
Cellular differentiation Lewis X Antigen Nerve Tissue Proteins Cell Separation Biology Stem cell marker Fatty Acid-Binding Proteins Mice Neural Stem Cells Antigens CD Neurosphere Fatty acid binding Animals AC133 Antigen Fluorescent Dyes Glycoproteins Neurons Medicine(all) Brain Cell Differentiation General Medicine Cell Biology FABP7 Molecular biology Embryonic stem cell Neural stem cell Cell biology Oligodendroglia Phenotype Astrocytes Fatty Acid-Binding Protein 7 Peptides Intracellular Protein Binding Developmental Biology |
| Zdroj: | Stem Cell Research. 11(3):1314-1322 |
| ISSN: | 1873-5061 |
| DOI: | 10.1016/j.scr.2013.09.002 |
| Popis: | Methods for the isolation of live neural stem cells from the brain are limited due to the lack of well-defined cell surface markers and tools to detect intracellular markers. To date most methods depend on the labeling of extracellular markers using antibodies, with intracellular markers remaining inaccessible in live cells. Using a novel intracellular protein FABP7 (Fatty Acid Binding Protein-7) selective fluorescent chemical probe CDr3, we have successfully isolated high FABP7 expressing cells from the embryonic and adult mouse brains. These cells are capable of forming neurospheres in culture, express neural stem cell marker genes and differentiate into neurons, astrocytes and oligodendrocytes. Characterization of cells sorted with Aldefluor or antibodies against CD133 or SSEA-1 showed that the cells isolated by CDr3 exhibit a phenotype distinct from the cells sorted with conventional methods. FABP7 labeling with CDr3 represents a novel method for rapid isolation of neural stem cells based on the expression of a single intracellular marker. |
| Databáze: | OpenAIRE |
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