Quantification of lectin in freshwater prawn (Macrobrachium rosenbergii) hemolymph by ELISA
| Autor: | Lorena Vázquez, Concepción Agundis, Edgar Zenteno, Colette Brassart, Claudia Sierra, Ali Pereyra, Roberto Zenteno |
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| Rok vydání: | 2000 |
| Předmět: |
Acetylgalactosamine
Time Factors Hemagglutination Physiology medicine.drug_class Enzyme-Linked Immunosorbent Assay Monoclonal antibody Biochemistry Acetylglucosamine Mice chemistry.chemical_compound Glucosamine Hemolymph Lectins Neuraminic acid medicine Animals Molecular Biology Hybridomas Dose-Response Relationship Drug biology Macrobrachium rosenbergii Antibodies Monoclonal Lectin biology.organism_classification Molecular biology N-Acetylneuraminic Acid chemistry Prawn biology.protein Palaemonidae |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 127:165-172 |
| ISSN: | 1096-4959 |
| DOI: | 10.1016/s0305-0491(00)00248-0 |
| Popis: | An enzyme-linked immunoabsorbent assay was developed to quantify the lectin present in the hemolymph of the freshwater prawn Macrobrachium rosenbergii. This method involves the use of murine monoclonal IgG1 with κ light chain (designated as 3G1) antibodies raised against the purified lectin, the assay that we developed recognized as little as 30 ng/ml of lectin, and was used to measure the lectin concentration in animals at different maturation stages. The highest concentration of lectin was identified in the hemolymph from post-larval prawns and the lowest in molt stage adult animals. The hemagglutination activity of the lectin was four-fold higher in adult than in juvenile specimens, although in all cases N-acetylated sugar residues, such as N-acetyl- d -glucosamine, N-acetyl- d -galactosamine, and N-acetyl- d -neuraminic acid were inhibitors of the lectin activity, suggesting that lectin plays a role in the transport of N-acetylated sugar in juvenile prawns. Our results indicate that lectin concentration and hemagglutinating activity could be influenced by developmental conditions of the freshwater prawn. |
| Databáze: | OpenAIRE |
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