An efficient and versatile approach for the construction of oligonucleotide microarrays
| Autor: | Mahajan Sk, K.C. Gupta, Pradeep Kumar |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Ethylene Oxide
Base Pair Mismatch Oligonucleotide Chemistry Organic Chemistry Clinical Biochemistry Oligonucleotides Pharmaceutical Science Biochemistry Combinatorial chemistry Molecular hybridization Oligonucleotide Microarrays Covalent bond Drug Discovery Epoxy Compounds Molecular Medicine Organic chemistry Glass DNA microarray Molecular Biology Oligonucleotide Array Sequence Analysis |
| Zdroj: | Bioorganic & Medicinal Chemistry Letters. 16:5654-5658 |
| ISSN: | 0960-894X |
| DOI: | 10.1016/j.bmcl.2006.08.014 |
| Popis: | A new immobilization chemistry for covalent attachment of phosphorylated oligonucleotides on epoxy-activated glass surface via opening of oxirane ring is described. The proposed strategy results in excellent immobilization efficiency, spot homogeneity, and morphology. The constructed microarray was successfully demonstrated for discrimination of nucleotide mismatches. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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