A bacterial artificial chromosome library for sequencing the complete human genome
| Autor: | Kazutoyo Osoegawa, Joseph J. Catanese, Aaron G. Mammoser, Chenyan Wu, Changjiang Zeng, Eirik Frengen, Pieter J. de Jong |
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| Rok vydání: | 2001 |
| Předmět: |
Genetic Markers
Chromosomes Artificial Bacterial Recombinant Fusion Proteins Molecular Sequence Data Restriction Mapping Biology DNA Satellite DNA sequencing Deoxyribonuclease EcoRI Sequence-tagged site Contig Mapping Human Genome Project Genetics Humans Genomic library Cloning Molecular Deoxyribonucleases Type II Site-Specific Genetics (clinical) Sequence Tagged Sites Gene Rearrangement Recombination Genetic Bacterial artificial chromosome Genomic Library Contig Donor selection Genome project Sequence Analysis DNA Resources Human genome |
| Zdroj: | Genome research. 11(3) |
| ISSN: | 1088-9051 |
| Popis: | A 30-fold redundant human bacterial artificial chromosome (BAC) library with a large average insert size (178 kb) has been constructed to provide the intermediate substrate for the international genome sequencing effort. The DNA was obtained from a single anonymous volunteer, whose identity was protected through a double-blind donor selection protocol. DNA fragments were generated by partial digestion with EcoRI (library segments 1–4: 24-fold) and MboI (segment 5: sixfold) and cloned into the pBACe3.6 and pTARBAC1 vectors, respectively. The quality of the library was assessed by extensive analysis of 169 clones for rearrangements and artifacts. Eighteen BACs (11%) revealed minor insert rearrangements, and none was chimeric. This BAC library, designated as “RPCI-11,” has been used widely as the central resource for insert-end sequencing, clone fingerprinting, high-throughput sequence analysis and as a source of mapped clones for diagnostic and functional studies.The sequence data described in this paper have been submitted to the GenBank data library under accession nos. AQ936150–AQ936491.] |
| Databáze: | OpenAIRE |
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