Structure of the human CpG-island-containing lung Kruppel-like factor (LKLF) gene and its location in chromosome 19p13.11–13 locus
| Autor: | Sergey V. Kozyrev, D A Domninsky, Andrew B. Poltaraus, Lise Lotte Hansen, Lev L. Kisselev |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
DNA
Complementary Molecular Sequence Data Kruppel-Like Transcription Factors Biophysics Locus (genetics) Biology Biochemistry Mice Exon Structural Biology Complementary DNA Genetics Animals Humans Amino Acid Sequence Molecular Biology Gene Peptide sequence Zinc finger transcription factor Zinc finger Base Sequence Sequence Homology Amino Acid Chromosome Mapping Zinc Fingers Sequence Analysis DNA Cell Biology Molecular biology NotI jumping clone CpG site Lung Kruppel-like factor gene Human chromosome 19 Trans-Activators Kruppel-like protein family CpG island CpG Islands Chromosomes Human Pair 19 |
| Zdroj: | FEBS Letters. (1):149-152 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/S0014-5793(99)00348-8 |
| Popis: | We have cloned earlier a short human genomic fragment which showed strong similarity with the mouse cDNA encoding lung Kruppel-like zinc finger transcription factor (LKLF), predominantly expressed in mouse developing lung, spleen, and vascular system, which might play a key role in programming the quiescent state of single positive T cells and blood vessel wall morphogenesis. Here we report the successful cloning of the human LKLF cDNA, its genomic structure and chromosomal localization at the 19p13.11–p13.13 locus. The full-length human LKLF cDNA has longer 5′-UTR with higher GC content than mouse cDNA and encodes a predicted protein of 355 amino acids which has three zinc fingers at the C-terminus and a proline-rich N-terminal domain. Human and mouse proteins share 87.3% identity and 90.2% amino acid similarity. The human LKLF gene consists of three exons. From the proximal promoter to the end of the second exon, we have found a CpG island with an average 76% GC content and two regions of unusually high GC density. |
| Databáze: | OpenAIRE |
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