Prophylactic Effects of Bee Venom Phospholipase A2 in Lipopolysaccharide-Induced Pregnancy Loss

Autor: Deok-Sang Hwang, Hyunsu Bae, Jin-Wook Lee, Na-Hoon Kang, HyeJin Yang, Jong Hoon Lee, Hyunjung Baek
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Lipopolysaccharides
Lipopolysaccharide
Fetal Resorption
Regulatory T cell
Health
Toxicology and Mutagenesis
Population
lcsh:Medicine
Tregs
Toxicology
T-Lymphocytes
Regulatory
Article
Proinflammatory cytokine
Andrology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Phospholipase A2
medicine
Animals
depletion of regulatory T cell (DEREG) mouse model
education
030304 developmental biology
0303 health sciences
education.field_of_study
Pregnancy
Fetus
030219 obstetrics & reproductive medicine
biology
business.industry
bee venom phospholipase A2
Uterus
lcsh:R
medicine.disease
Abortion
Spontaneous
Killer Cells
Natural
Mice
Inbred C57BL
Bee Venoms
Phospholipases A2
medicine.anatomical_structure
spontaneous abortion
chemistry
embryonic structures
biology.protein
Cytokines
Female
pregnancy
business
Zdroj: Toxins, Vol 11, Iss 7, p 404 (2019)
Toxins
Volume 11
Issue 7
ISSN: 2072-6651
Popis: Spontaneous abortion represents a common form of embryonic loss caused by early pregnancy failure. In the present study, we investigated the prophylactic effects of bee venom phospholipase A2 (bvPLA2), a regulatory T cell (Treg) inducer, on a lipopolysaccharide (LPS)-induced abortion mouse model. Fetal loss, including viable implants, the fetal resorption rate, and the fetal weight, were measured after LPS and bvPLA2 treatment. The levels of serum and tissue inflammatory cytokines were determined. To investigate the involvement of the Treg population in bvPLA2-mediated protection against fetal loss, the effect of Treg depletion was evaluated following bvPLA2 and LPS treatment. The results clearly revealed that bvPLA2 can prevent fetal loss accompanied by growth restriction in the remaining viable fetus. When the LPS-induced abortion mice were treated with bvPLA2, Treg cells were significantly increased compared with those in the non-pregnant, PBS, and LPS groups. After LPS injection, the levels of proinflammatory cytokines were markedly increased compared with those in the PBS mouse group, while bvPLA2 treatment showed significantly decreased TNF-&alpha
and IFN-&gamma
expression compared with that in the LPS group. The protective effects of bvPLA2 treatment were not detected in Treg-depleted abortion-prone mice. These findings suggest that bvPLA2 has protective effects in the LPS-induced abortion mouse model by regulating Treg populations.
Databáze: OpenAIRE
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