Prophylactic Effects of Bee Venom Phospholipase A2 in Lipopolysaccharide-Induced Pregnancy Loss
Autor: | Deok-Sang Hwang, Hyunsu Bae, Jin-Wook Lee, Na-Hoon Kang, HyeJin Yang, Jong Hoon Lee, Hyunjung Baek |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Lipopolysaccharides
Lipopolysaccharide Fetal Resorption Regulatory T cell Health Toxicology and Mutagenesis Population lcsh:Medicine Tregs Toxicology T-Lymphocytes Regulatory Article Proinflammatory cytokine Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Phospholipase A2 medicine Animals depletion of regulatory T cell (DEREG) mouse model education 030304 developmental biology 0303 health sciences education.field_of_study Pregnancy Fetus 030219 obstetrics & reproductive medicine biology business.industry bee venom phospholipase A2 Uterus lcsh:R medicine.disease Abortion Spontaneous Killer Cells Natural Mice Inbred C57BL Bee Venoms Phospholipases A2 medicine.anatomical_structure spontaneous abortion chemistry embryonic structures biology.protein Cytokines Female pregnancy business |
Zdroj: | Toxins, Vol 11, Iss 7, p 404 (2019) Toxins Volume 11 Issue 7 |
ISSN: | 2072-6651 |
Popis: | Spontaneous abortion represents a common form of embryonic loss caused by early pregnancy failure. In the present study, we investigated the prophylactic effects of bee venom phospholipase A2 (bvPLA2), a regulatory T cell (Treg) inducer, on a lipopolysaccharide (LPS)-induced abortion mouse model. Fetal loss, including viable implants, the fetal resorption rate, and the fetal weight, were measured after LPS and bvPLA2 treatment. The levels of serum and tissue inflammatory cytokines were determined. To investigate the involvement of the Treg population in bvPLA2-mediated protection against fetal loss, the effect of Treg depletion was evaluated following bvPLA2 and LPS treatment. The results clearly revealed that bvPLA2 can prevent fetal loss accompanied by growth restriction in the remaining viable fetus. When the LPS-induced abortion mice were treated with bvPLA2, Treg cells were significantly increased compared with those in the non-pregnant, PBS, and LPS groups. After LPS injection, the levels of proinflammatory cytokines were markedly increased compared with those in the PBS mouse group, while bvPLA2 treatment showed significantly decreased TNF-&alpha and IFN-&gamma expression compared with that in the LPS group. The protective effects of bvPLA2 treatment were not detected in Treg-depleted abortion-prone mice. These findings suggest that bvPLA2 has protective effects in the LPS-induced abortion mouse model by regulating Treg populations. |
Databáze: | OpenAIRE |
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