BioID identifies proteins involved in the cell biology of caveolae
| Autor: | Kirsi Riento, Carolina Mendoza-Topaz, Benjamin J. Nichols, Ivana Yeow |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
rac1 GTP-Binding Protein B Vitamins Cell Membranes Immunofluorescence Cultured tumor cells Biochemistry Madin Darby Canine Kidney Cells 0302 clinical medicine Caveolae Small GTPase Small interfering RNAs RNA Small Interfering Multidisciplinary Chemistry Organic Compounds Signal transducing adaptor protein Vitamins Cell biology Precipitation Techniques DNA-Binding Proteins Nucleic acids Biotinylation Physical Sciences Cell lines Medicine Cellular Structures and Organelles Biological cultures Research Article Immunoprecipitation Science Biotin RAC1 03 medical and health sciences Dogs Genetics Animals Humans HeLa cells Non-coding RNA Immunoassays Adaptor Proteins Signal Transducing Organic Chemistry Chemical Compounds RNA Biology and Life Sciences Membrane Proteins Cell Biology Cell cultures Gene regulation Research and analysis methods Cytoskeletal Proteins 030104 developmental biology Membrane protein Coated Pits Immunologic Techniques Gene expression 030217 neurology & neurosurgery |
| Zdroj: | PLoS ONE, Vol 13, Iss 12, p e0209856 (2018) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The mechanisms controlling the abundance and sub-cellular distribution of caveolae are not well described. A first step towards determining such mechanisms would be identification of relevant proteins that interact with known components of caveolae. Here, we applied proximity biotinylation (BioID) to identify a list of proteins that may interact with the caveolar protein cavin1. Screening of these candidates using siRNA to reduce their expression revealed that one of them, CSDE1, regulates the levels of mRNAs and protein expression for multiple components of caveolae. A second candidate, CD2AP, co-precipitated with cavin1. Caveolar proteins were observed in characteristic and previously un-described linear arrays adjacent to cell-cell junctions in both MDCK cells, and in HeLa cells overexpressing an active form of the small GTPase Rac1. CD2AP was required for the recruitment of caveolar proteins to these linear arrays. We conclude that BioID will be useful in identification of new proteins involved in the cell biology of caveolae, and that interaction between CD2AP and cavin1 may have an important role in regulating the sub-cellular distribution of caveolae. |
| Databáze: | OpenAIRE |
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