Molecular detection and characterization of Ehrlichia ruminantium from cattle in Mozambique
| Autor: | Rosangela Zacarias Machado, Luiz Ricardo Gonçalves, Natalia Serra Mendes, Inalda Angélica de Souza Ramos, Diego Carlos de Souza Zanatto, Carlos Antonio Matos, Marcos Rogério André |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Direcção de Ciências Animais |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
animal structures Veterinary (miscellaneous) 030231 tropical medicine Cattle Diseases Ehrlichia ruminantium pCS20 Polymerase Chain Reaction Heartwater (cowdriosis) 03 medical and health sciences 0302 clinical medicine Maputo Province Genotype Parasite hosting Animals map1 Gene Mozambique Phylogeny DNA Primers Genetics Genetic diversity biology Phylogenetic tree Base Sequence Ehrlichia Genetic Variation DNA Ruminants 030108 mycology & parasitology Amplicon biology.organism_classification Infectious Diseases PCR Insect Science Africa Parasitology Cattle Heartwater Disease |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1873-6254 |
| Popis: | Made available in DSpace on 2019-10-06T16:13:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-03-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Heartwater caused by Ehrlichia ruminantiumis a disease of domestic and wild ruminants and one of the most economically important tick-borne diseases in Africa. The present study aimed to investigate the occurrence and genetic diversity of E. ruminantium in blood samples from 210 cattle sampled in five districts of Maputo Province, Mozambique. DNA blood samples were initially submitted to PCR assays targeting E. ruminantium pCS20 gene fragments. Additionally, in order to assess the genetic diversity of E. ruminantium, the positive samples were submitted to a PCR assay targeting the E. ruminantium map1 gene. Finally, the amplicons were sequenced and phylogenetic position was inferred using the Maximum Likelihood method. PCR results revealed that the overall prevalence in Maputo Province was 15% of the animals sampled. E. ruminantium map1 sequences showed not to be conserved. In the phylogenetic analysis, E. ruminantium map1 genotypes were positioned into multiple-clades. This study provides information on the prevalence and genetic diversity of E. ruminantium in five localities of Maputo Province. The future immune control strategies against local E. ruminantium must be designed in the light of the genetic diversity of this parasite. Laboratório de Imunoparasitologia Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (Unesp) Direcção de Ciências Animais Laboratório de Imunoparasitologia Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (Unesp) FAPESP: 2012 ̸ 213714 |
| Databáze: | OpenAIRE |
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