| Autor: |
Kârlis Žagata, Reinis Ornicāns, Oksana Fokina, Nadežda Ļisina, Dace Grauda, Isaak Rashal, Andra Miķelsone, Lita Lapiņa |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Proceedings of the Latvian Academy of Sciences. Section B, Natural Sciences, Vol 68, Iss 3-4, Pp 142-147 (2014) |
| ISSN: |
1407-009X |
| DOI: |
10.2478/prolas-2014-0016 |
| Popis: |
Our goal was to improve the method of obtaining double haploids (DH) by anther culture from Latvian breeding material for several agriculturally important cereals in Latvia: barley, spring and winter wheat. Hybrids from Latvian breeders were initial material. It was found that copper (2.5 mg/l) added to barley anther pre-treatment media and to both barley (C3) and wheat (liquid AMC) induction media, resulted in a higher percentage of green plants-regenerants. In general, the winter wheat hybrids exhibited lower embryogenesis than spring wheat hybrids. Obtaining a large number of DH lines from barley and wheat hybrids with unknown androgenesis response can be organized in two stages: first - selection from breeding initial material hybrids responsive in anther culture, which enables production of green plants-regenerants, and second - production of DH lines in a large scale from selected hybrids. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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