Effects of two infectious bursal disease vaccine virus strains on hepatic microsomal enzyme activities in chickens
| Autor: | Andreja Prevendar Crnić, Stanislav Čajavec, Zdenko Biđin, Darko Sakar, Ivana Lojkić, Jelena Pompe-Gotal |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Reductase Antibodies Viral Vaccines Attenuated Infectious bursal disease virus Virus Microbiology Infectious bursal disease Random Allocation infectious bursal disease vaccines hepatic biotransformation enzymes broiler chickens medicine Animals Poultry Diseases chemistry.chemical_classification Attenuated vaccine General Veterinary biology Viral Vaccine Vaccination Viral Vaccines Birnaviridae Infections medicine.disease Virology Enzyme Animals Newborn chemistry Microsomes Liver biology.protein Female Antibody Chickens |
| Zdroj: | Acta Veterinaria Hungarica. 54:281-293 |
| ISSN: | 1588-2705 0236-6290 |
| DOI: | 10.1556/avet.54.2006.2.14 |
| Popis: | The influence of two infectious bursal disease vaccines on the activities of hepatic microsomal enzymes aniline hydroxylase, ethylmorphine N-demethylase, NADPH-cytochrome c reductase, aryl sulphotransferase and p-nitrophenol UDP-glucuronyltransferase was investigated in chickens. The vaccines contained attenuated Winterfield 2512 and VMG-91 strains, respectively. The activities of enzymes were determined on postvaccination days 0, 2, 5 and 7. At the same time, post-mitochondrial supernatant, cytosolic and microsomal pellet protein concentrations were determined. As expected, the antibody titres against infectious bursal disease virus in the serum were increased in both tested groups in relation to each administered vaccine. Using RT-PCR, the presence of the VP2 gene fragment of virus in the liver of chicken was demonstrated 4 and 6 h after vaccination. The results of this study suggest that the two commercial vaccines modulate the activities of five enzymes tested, and that the two attenuated vaccines applied triggered induction and/or inhibition of phases I and II of biotransformation enzyme activities. |
| Databáze: | OpenAIRE |
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