The changes in the transcriptomic profiling of subcutaneous adipose tissue after bariatric surgery depend on the insulin resistance state
| Autor: | Alberto Rodríguez-Cañete, Francisca Rodriguez-Pacheco, Lourdes Garrido-Sánchez, Francisco J. Moreno-Ruiz, Ailec Ho-Plagaro, Eduardo García-Fuentes, Sara García-Serrano, Juan José González-Plaza, Carolina Gutiérrez-Repiso, Concepcion Santiago-Fernandez |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Chemokine Subcutaneous Fat Bariatric Surgery Inflammation Cohort Studies 03 medical and health sciences chemistry.chemical_compound Insulin resistance medicine Humans Phosphatidylinositol Oligonucleotide Array Sequence Analysis biology Microarray analysis techniques Kinase business.industry Gene Expression Profiling Glycosaminoglycan metabolic process medicine.disease Obesity Morbid Surgery 030104 developmental biology chemistry biology.protein Female sense organs Insulin Resistance medicine.symptom Transcriptome Janus kinase business |
| Zdroj: | Surgery for Obesity and Related Diseases. 14:1182-1191 |
| ISSN: | 1550-7289 |
| DOI: | 10.1016/j.soard.2018.04.010 |
| Popis: | Background The changes that are produced in the gene expression of subcutaneous adipose tissue after Roux-en-Y gastric bypass are not yet fully known. Objective To identify the changes in the subcutaneous adipose tissue gene expression of morbidly obese women with low insulin resistance (MO-low-IR) and high insulin resistance (MO-high-IR) to find a relationship with measured obesity-related co-morbidities. Setting A university hospital. Methods Subcutaneous adipose tissue samples were assessed by microarray analysis before and 2 years after Roux-en-Y gastric bypass in MO-low-IR and MO-high-IR patients. Results There is a group of shared differentially expressed genes (DEG) in both MO-low-IR and MO-high-IR, also there is a group of exclusive DEG in MO-low-IR and another group in MO-high-IR. In MO-high-IR, the downexpressed DEG are related to the regulation of transcription and are involved in the pathways related to cytokine-cytokine receptor interaction, cancer, phosphatidylinositol 3-kinase-protein kinase B signaling, human T-lymphotropic virus I infection, chemokine signaling, and Janus kinase/signal transducers and activators of transcription signaling. In MO-low-IR, the overexpressed DEG are related to carbohydrate metabolic processes, the downexpressed DEG to the glycosaminoglycan metabolic process and regulation of translation, and the pathways are related to phosphatidylinositol 3-kinase-protein kinase B signaling and metabolic pathways. The fold change of DEG mainly correlates with the percentage of change (Δ) of waist, Δhip, Δglucose, and Δtriglycerides. These DEG were mainly related to cancer, inflammation/immune regulation, metabolic pathways, ribonucleic acid/deoxyribonucleic acid regulation, virus infection, and regulation of cellular proliferation. Conclusions This study suggests a potential association between high insulin resistance and the expression of genes related to cancer and chronic immune activation/inflammation. |
| Databáze: | OpenAIRE |
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