Direct real-time measurement of intra-oocyte nitric oxide concentration in vivo
| Autor: | A.P. Goud, Bernard Gonik, Husam M. Abu-Soud, Pravin T. Goud, Ghassan M. Saed, Michael P. Diamond, Tohid Najafi, Xueji Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
lcsh:Medicine
Nitric Oxide Biochemistry 01 natural sciences Nitric oxide Mice 03 medical and health sciences chemistry.chemical_compound Human fertilization In vivo medicine Animals lcsh:Science Electrodes Incubation 030304 developmental biology 0303 health sciences Multidisciplinary Extramural 010401 analytical chemistry Embryogenesis lcsh:R Biology and Life Sciences Oocyte activation Cell Biology Oocyte 0104 chemical sciences Cell biology medicine.anatomical_structure chemistry Fertilization Calibration Cytochemistry Oocytes Female lcsh:Q Research Article Developmental Biology |
| Zdroj: | PLoS ONE, Vol 9, Iss 6, p e98720 (2014) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Nitric oxide (NO) is reported to play significant a role in oocyte activation and maturation, implantation, and early embryonic development. Previously we have shown that NO forms an important component of the oocyte microenvironment, and functions effectively to delay oocyte aging. Thus, precise information about intra-oocyte NO concentrations [NO] will result in designing more accurate treatment plans in assisted reproduction. In this work, the direct, real-time and quantitative intra-oocyte [NO] was measured utilizing an L-shaped amperometric integrated NO-selective electrode. This method not only provides an elegant and convenient approach to real-time the measurement of NO in physiological environments, but also mimics the loss of NO caused by rapid NO diffusion combined with its reactivity in the biological milieu. This experiment suggests that the NO levels of oocytes obtained from young animals are significantly higher than those of oocytes obtained from old animals. Additionally the NO levels stay constant during the measurements; however, the intra-oocyte [NO] is reduced significantly (70-75% reduction) in response to L-NAME incubation, suggesting that NO measurements are truly NOS based rather than caused by an unknown interfering substance in our system. We believe this first demonstration of the direct quantitative measurement of [NO] in situ in an intact cellular complex should be useful in tracking real-time and rapid changes at nanomolar levels. Moreover, this finding confirms and extends our previous work showing that supplementation with NO delays the oocyte aging process. |
| Databáze: | OpenAIRE |
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