Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneol-producing cyanobacterium Pseudanabaena galeata
| Autor: | Maki Kawai-Yamada, Masatoshi Yamaguchi, Takashi Asaeda, Motonobu Miyazaki, Atsuko Miyagi, Toshiki Ishikawa, Hirofumi Uchimiya, Kazuaki Saito, Masayuki Kakimoto |
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| Rok vydání: | 2014 |
| Předmět: |
Cyanobacteria
Camphanes biology Physiology Metabolite Temperature Primary metabolite Gene Expression Regulation Bacterial Plant Science biology.organism_classification digestive system diseases Citric acid cycle Metabolic pathway chemistry.chemical_compound Biochemistry Biosynthesis chemistry Odor Gene expression neoplasms Agronomy and Crop Science Metabolic Networks and Pathways |
| Zdroj: | Journal of Plant Physiology. 171:292-300 |
| ISSN: | 0176-1617 |
| DOI: | 10.1016/j.jplph.2013.09.005 |
| Popis: | A volatile metabolite, 2-methylisoborneol (2-MIB), causes an unpleasant taste and odor in tap water. Some filamentous cyanobacteria produce 2-MIB via a two-step biosynthetic pathway: methylation of geranyl diphosphate (GPP) by methyl transferase (GPPMT), followed by the cyclization of methyl-GPP by monoterpene cyclase (MIBS). We isolated the genes encoding GPPMT and MIBS from Pseudanabaena galeata, a filamentous cyanobacterium known to be a major causal organism of 2-MIB production in Japanese lakes. The predicted amino acid sequence showed high similarity with that of Pseudanabaena limnetica (96% identity in GPPMT and 97% identity in MIBS). P. galeata was cultured at different temperatures to examine the effect of growth conditions on the production of 2-MIB and major metabolites. Gas chromatograph-mass spectrometry (GC-MS) measurements showed higher accumulation of 2-MIB at 30 °C than at 4 °C or 20 °C after 24 h of culture. Real-time-RT PCR analysis showed that the expression levels of the genes encoding GPPMT and MIBS decreased at 4 °C and increased at 30 °C, compared with at 20 °C. Furthermore, metabolite analysis showed dramatic changes in primary metabolite concentrations in cyanobacteria grown at different temperatures. The data indicate that changes in carbon flow in the TCA cycle affect 2-MIB biosynthesis at higher temperatures. |
| Databáze: | OpenAIRE |
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