Impairment of spatial working memory and oxidative stress induced by repeated crack cocaine inhalation in rats

Autor: Elisa Fraga Gomes, Ester Miyuki Nakamura Palacios, Rita Gomes Wanderley Pires, Cristina Martins e Silva, Ingryd Fortes Souza Lipaus, Cleciane Waldetário Martins, Fernanda Malgarin, Patrícia F. Schuck, Lívia Carla de Melo Rodrigues
Rok vydání: 2019
Předmět:
Male
medicine.medical_specialty
Antioxidant
medicine.medical_treatment
Hippocampus
medicine.disease_cause
Gas Chromatography-Mass Spectrometry
Lipid peroxidation
Superoxide dismutase
03 medical and health sciences
Behavioral Neuroscience
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
Administration
Inhalation
TBARS
Animals
Medicine
Rats
Wistar
Maze Learning
030304 developmental biology
chemistry.chemical_classification
Glutathione Peroxidase
Memory Disorders
0303 health sciences
Inhalation
biology
Superoxide Dismutase
business.industry
Glutathione peroxidase
Catalase
Rats
Disease Models
Animal
Oxidative Stress
Memory
Short-Term
Endocrinology
Advanced Oxidation Protein Products
chemistry
biology.protein
Crack Cocaine
Lipid Peroxidation
business
030217 neurology & neurosurgery
Oxidative stress
Zdroj: Behavioural Brain Research. 359:910-917
ISSN: 0166-4328
Popis: Crack cocaine is a highly toxic drug with great potential to induce addiction. It produces more intense effects than cocaine powder, with its use having grown worldwide. However, few studies have focused on the cognitive and biochemical consequences that result from crack cocaine inhalation. This study examined the effects of direct crack cocaine inhalation on spatial working memory and brain oxidative stress parameters in rats. Male adult Wistar rats, well-trained in an eight-arm radial maze (8-RM), underwent five sessions of crack cocaine inhalation (crack cocaine group) once a day or inhalation simulation (sham group), being tested in 1-h delayed tasks 24 h after the last inhalation. An additional inhalation session was carried out the following day, with the prefrontal cortex, hippocampus and striatum being removed five minutes afterwards in order to assess oxidative damage such as lipid peroxidation, thiobarbituric acid-reactive species (TBARS) levels, and advanced oxidation protein products (AOPP), as well as the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Animals from the crack cocaine group showed more errors (p 0.01) in the 1-h post-delay performance in the 8-RM when compared to the sham group. The crack cocaine group showed decreased (p 0.05) lipid peroxidation in the hippocampus and increased (p 0.001) levels of AOPP and SOD activity (p 0.05) in the striatum when compared to the sham group. Therefore, the repeated inhalation of crack cocaine impaired long-term spatial working memory and elicited oxidative stress in the hippocampus and striatum of rats.
Databáze: OpenAIRE
Externí odkaz: