Dietary protein:lipid ratio modulates somatic growth and expression of genes involved in somatic growth, lipid metabolism and food intake in Pejerrey fry (Odontesthes bonariensis)

Autor: Juan Ignacio Bertucci, Ayelén Melisa Blanco, Juan Carlos Navarro, Suraj Unniappan, Luis Fabián Canosa
Přispěvatelé: Agencia Nacional de Promoción Científica y Tecnológica (Argentina), Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina), Natural Sciences and Engineering Research Council of Canada, Canadian Institutes of Health Research, Government of Canada
Rok vydání: 2022
Předmět:
Zdroj: Comparative biochemistry and physiology. Part A, Molecularintegrative physiology. 270
ISSN: 1531-4332
Popis: Pejerrey is a freshwater fish from South America with high potential for aquaculture. This study was designed to determine the effects of different dietary protein:lipid ratio on growth rate and the expression of growth, lipid metabolism and feeding-related genes of this species during early developmental stages. Pejerrey fry were fed for 60 days with four experimental diets containing low (400 g Kg) or high (500 g Kg) protein (LP or HP, respectively) and low (120 g Kg) or high (200 g Kg) lipid (LL or HL, respectively), in the combinations: LP-LL; LP-HL; HP-LL and HP-HL. Measurements of growth, lipid and fatty acid content of fry, expression of genes from the endocrine axis (gh, ghrs, igfs), fatty acid metabolism (∆6-desaturase), and food intake behavior (nucb2/nesfatin-1) were collected. Fry fed with diets LP-LL and HP-LL showed the highest growth rate and growth hormone (gh) mRNA expression levels. The gene expression of ∆6-desaturase was high in head of fry fed with diet LP-HL. The mRNA expression of nucb2/nesfatin-1 and gh followed the same patterns in head, and the inverse pattern in body. In conclusion, diets with LL ensure a higher growth of pejerrey fry compared to those that contain HL, without altering the final lipid amount nor the fatty acid profile on fry. In LL groups, the expression of genes from the GH-IGF axis is associated with the observed promotion of somatic growth. The expression of nucb2/nesfatin-1 indicates an effect of this peptide not related to food intake regulation, e.g., a negative regulatory role on GH expression, that would warrant future research.
This work was partially funded by Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT, Argentina; PICT 2010–1493) and Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET; PIP 2014/0595) to LFC, and a Discovery grant, and Discovery Accelerator Supplement award from the Natural Sciences and Engineering Research Council (NSERC) of Canada to SU. SU received a new investigator salary award from the Canadian Institutes of Health Research (CIHR). JIB was recipient of a doctoral fellowship from ANPCyT (Argentina), and from the Emerging Leaders of the Americas Program (ELAP) scholarship from the Government of Canada and doctoral fellow from CONICET (Argentina).
Databáze: OpenAIRE
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