Dissolving microneedle based transdermal delivery of therapeutic peptide analogues
| Autor: | Peter McLoughlin, Niall J. O’Reilly, Chris J. Allender, Colin Dillon, David Anthony Barrow, Helen Hughes |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
Microinjections Swine Skin Absorption Pharmaceutical Science Peptide 02 engineering and technology Administration Cutaneous 030226 pharmacology & pharmacy Sincalide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Drug Delivery Systems Peptide synthesis medicine Animals Porcine skin Transdermal Polymyxin B Skin chemistry.chemical_classification Permeation 021001 nanoscience & nanotechnology Combinatorial chemistry chemistry Solubility Needles Skin penetration Female Pentagastrin 0210 nano-technology Peptide drug medicine.drug |
| Zdroj: | International journal of pharmaceutics. 565 |
| ISSN: | 1873-3476 |
| Popis: | Microneedle technology offers a viable means of delivering biologically active pharmaceutical agents across the skin in a minimally invasive and virtually pain free manner. Previous work detailed the first successful transdermal delivery of a model peptide drug, polymyxin b, utilising a dissolving polymer-based microneedle system. The focus of this study was to examine the ability of a dissolving microneedle system to deliver a range of peptides of different sizes and properties. Analogue versions of 2 existing therapeutic peptides; pentagastrin and sincalide, were synthesised utilising Fmoc based solid phase peptide synthesis (SPPS) chemistry techniques and once successfully synthesised and purified, the peptide analogues were characterised using LC-MS. The peptide analogues were then incorporated into PVP/trehalose microneedle formulations. Skin permeation testing, in addition to skin penetration testing, was carried out to determine the effectiveness of the microneedle system to deliver the peptide analogues through porcine skin. The results obtained from these studies were then compared with the permeation results obtained utilising polymyxin B as the peptide drug cargo to evaluate the PVP/trehalose microneedle system's suitability to successfully deliver therapeutic peptides. Results indicated that the microneedle system successfully systemically delivered a higher overall percentage of the encapsulated peptides at an initially faster rate than peptide loaded control discs and in therapeutically relevant concentrations. |
| Databáze: | OpenAIRE |
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