Generative cells ofLilium longiflorum possess translatable mRNA and functional protein synthesis machinery
| Autor: | Mohan Singh, Cecilia K. Blomstedt, Robert Bruce Knox |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Plant Development
Cell Separation Plant Science Cycloheximide Biology chemistry.chemical_compound Gene expression Genetics medicine Protein biosynthesis RNA Messenger Nucleic Acid Synthesis Inhibitors Plant Proteins Protein Synthesis Inhibitors Messenger RNA Dactinomycin Stem Cells RNA Translation (biology) General Medicine Cell biology chemistry RNA Plant Protein Biosynthesis Pollen Stem cell Agronomy and Crop Science medicine.drug |
| Zdroj: | Plant Molecular Biology. 31:1083-1086 |
| ISSN: | 1573-5028 0167-4412 |
| Popis: | Metabolic labelling with [35S]-methionine demonstrated that generative cells of Lilium longiflorum possess their own set of mRNA and are capable of synthesising proteins independently from the vegetative cell. The isolated generative cells synthesised ten proteins, of which six were unique to these specialised cells. Isolation of generative cells from pollen grains after [35S]-methionine labelling resulted in an identical protein profile, therefore the synthesis of these proteins was not due to isolation shock. Addition of cycloheximide, abolished TCA-precipitable counts, whilst actinomycin D had no qualitative effect on the observed protein profile, indicating active translation of pre-existing mRNAs by the generative cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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