miRNA repression of translation in vitro takes place during 43S ribosomal scanning
| Autor: | Taran Limousin, Paulina S. Rubilar, Emiliano P. Ricci, Ricardo Soto-Rifo, Théophile Ohlmann, Didier Decimo |
|---|---|
| Přispěvatelé: | Université de Lyon, Virologie humaine, École normale supérieure de Lyon (ENS de Lyon)-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
MESH: Peptide Chain Initiation
Translational Proteasome Endopeptidase Complex [SDV]Life Sciences [q-bio] RNA Stability Hepacivirus Biology MESH: Ribosome Subunits Small Eukaryotic Ribosome Poly(A)-Binding Proteins MESH: Poly(A)-Binding Proteins chemistry.chemical_compound Poly(A)-binding protein Genetics Protein biosynthesis MESH: Hepacivirus RNA Messenger Peptide Chain Initiation Translational Psychological repression MESH: RNA Messenger Regulation of gene expression Ribosome Subunits Small Eukaryotic Eukaryotic Large Ribosomal Subunit EIF4G MESH: Peptides MESH: Proteasome Endopeptidase Complex Translation (biology) MESH: RNA Stability MESH: Eukaryotic Initiation Factor-4G Ribosome Subunits Large Eukaryotic MESH: Gene Expression Regulation Cell biology MicroRNAs chemistry MESH: Ribosome Subunits Large Eukaryotic Gene Expression Regulation MESH: Protein Biosynthesis Protein Biosynthesis biology.protein MESH: 5' Untranslated Regions RNA 5' Untranslated Regions Eukaryotic Initiation Factor-4G Peptides MESH: MicroRNAs |
| Zdroj: | Nucleic Acids Research Nucleic Acids Research, 2013, 41 (1), pp.586-98. ⟨10.1093/nar/gks1076⟩ |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gks1076⟩ |
| Popis: | International audience; microRNAs (miRNAs) regulate gene expression at multiple levels by repressing translation, stimulating deadenylation and inducing the premature decay of target messenger RNAs (mRNAs). Although the mechanism by which miRNAs repress translation has been widely studied, the precise step targeted and the molecular insights of such repression are still evasive. Here, we have used our newly designed in vitro system, which allows to study miRNA effect on translation independently of deadenylation. By using specific inhibitors of various stages of protein synthesis, we first show that miRNAs target exclusively the early steps of translation with no effect on 60S ribosomal subunit joining, elongation or termination. Then, by using viral proteases and IRES-driven mRNA constructs, we found that translational inhibition takes place during 43S ribosomal scanning and requires both the poly(A) binding protein and eIF4G independently from their physical interaction. |
| Databáze: | OpenAIRE |
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