Role of IL-24 in the mucosal remodeling of children with coeliac disease
| Autor: | Réka Rokonay, Attila Szabo, Beáta Szebeni, Rita Lippai, Domonkos Pap, Nóra Judit Béres, Gábor Veres, Ádám Vannay, Apor Veres-Székely |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Adolescent lcsh:Medicine Peripheral blood mononuclear cell General Biochemistry Genetics and Molecular Biology Coeliac disease 03 medical and health sciences Tissue remodeling 0302 clinical medicine Internal medicine medicine Humans Interleukin 20 subfamily Intestinal Mucosa Receptor Peripheral blood mononuclear cells (PBMC) Child Myofibroblasts Myofibroblast biology business.industry Research Interleukins lcsh:R General Medicine Hydrogen Peroxide medicine.disease Epithelium Epithelial cell Celiac Disease Interleukin 24 030104 developmental biology Endocrinology medicine.anatomical_structure IL1A Apoptosis 030220 oncology & carcinogenesis Child Preschool biology.protein Leukocytes Mononuclear Tumor necrosis factor alpha ACTA2 business |
| Zdroj: | Journal of Translational Medicine, Vol 18, Iss 1, Pp 1-13 (2020) Journal of Translational Medicine |
| ISSN: | 1479-5876 |
| Popis: | Background Recently, involvement of IL-19, IL-20 and IL-24 has been reported in inflammatory diseases associated with tissue remodeling. However, their impact on the pathomechanism of coeliac disease (CD) is still completely unknown. Methods Expression of IL19, IL20 and IL24 was measured by real-time RT-PCR, protein amount of IL-24, α smooth muscle actin (α-SMA) and fibronectin (FN) was determined by Western-blot analysis in the duodenal biopsies of therapy naive children with CD and controls. Localization of IL-24 and IL-20RB was investigated by immunofluorescent staining in the duodenal mucosa. Effect of recombinant IL-1β, TNF-α, TGF-β and IL-17 treatment on the expression of IL19, IL20, IL24 and their receptors was investigated by real-time RT-PCR in small intestinal epithelial cells (FHs74Int), in primary duodenal myofibroblasts (pdMFs) and in peripheral blood mononuclear cells (PBMCs). Effect of IL-24 on H2O2 treated FHs74Int cells and on pdMFs was measured by MTT, LDH, Annexin V assays, real-time RT-PCR and by fluorescent microscopy. Results We found increased level of IL-24 (3.3×, p IL19 (3.6×, p IL24 (5.2×, p IL24 expression of FHs74Int cells (9.9×, p IL1A, IL6 and TNF of H2O2-treated FHs74Int cells. IL-24 decreased the proliferation (0.6×, p ACTA2, ACTB, VIM, SNAI1 and SNAI2. Conclusion Our results suggest that IL-24 plays a significant role in the maintenance of duodenal mucosal integrity in CD. |
| Databáze: | OpenAIRE |
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