Peptoids advance multidisciplinary research and undergraduate education in parallel: Sequence effects on conformation and lipid interactions
| Autor: | Ann McMonigal, Andre Payman, Gillian E. Gadbois, Lauren Verheyden, Molly McGeehan, Shannon Chan, Jiacheng Tan, Samantha A. Parker, Kalli M. Dowell, Kira L. Palazzo, Grace Y. Stokes, Maritza Soria, Jennifer Yin, Jaenic J. Lee, Christian J. Jimenez, Amelia A. Fuller, Mikaela C. Ley, Cameron A. Read, Anna L. Calkins, Anmol Jandaur, Jesus E. Cervantes, Vivian T. Vo, Nicole Burgess, Tara Karanik |
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| Rok vydání: | 2018 |
| Předmět: |
Full Paper
Peptidomimetic Organic Chemistry Undergraduate education Biophysics Molecular Conformation Nanotechnology Peptoid Sequence (biology) General Medicine Glycerophospholipids Hydrogen-Ion Concentration Full Papers fluorescence spectroscopy Biochemistry phospholipid membranes Biomaterials chemistry.chemical_compound Peptoids Spectrometry Fluorescence chemistry peptidomimetics chemistry education sequence‐structure relationships |
| Zdroj: | Biopolymers |
| ISSN: | 1097-0282 |
| Popis: | Peptoids are versatile peptidomimetic molecules with wide‐ranging applications from drug discovery to materials science. An understanding of peptoid sequence features that contribute to both their three‐dimensional structures and their interactions with lipids will expand functions of peptoids in varied fields. Furthermore, these topics capture the enthusiasm of undergraduate students who prepare and study diverse peptoids in laboratory coursework and/or in faculty led research. Here, we present the synthesis and study of 21 peptoids with varied functionality, including 19 tripeptoids and 2 longer oligomers. We observed differences in fluorescence spectral features for 10 of the tripeptoids that correlated with peptoid flexibility and relative positioning of chromophores. Interactions of representative peptoids with sonicated glycerophospholipid vesicles were also evaluated using fluorescence spectroscopy. We observed evidence of conformational changes effected by lipids for select peptoids. We also summarize our experiences engaging students in peptoid‐based projects to advance both research and undergraduate educational objectives in parallel. |
| Databáze: | OpenAIRE |
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