Lipase from Brassica napus L. discriminates against cis-4 and cis-6 unsaturated fatty acids and secondary and tertiary alcohols
| Autor: | Kumar D. Mukherjee, Matthew J. Hills, Irmgard Kiewitt |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Biophysics
Brassica Biochemistry Substrate Specificity chemistry.chemical_compound Endocrinology Organic chemistry Triolein Lipase Triglycerides Unsaturated fatty acid chemistry.chemical_classification Petroselinic acid Esterification biology Chemistry Hydrolysis Fatty acid Kinetics Oleic acid Docosahexaenoic acid Alcohols Fatty Acids Unsaturated biology.protein Polyunsaturated fatty acid |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 1042:237-240 |
| ISSN: | 0005-2760 |
| DOI: | 10.1016/0005-2760(90)90014-o |
| Popis: | Lipase (EC 3.1.1.3) from oilseed rape (Brassica napus L., cv Ceres) hydrolyzes triacylglycerols containing a broad range of fatty acids at similar rates. In esterification reactions carried out in hexane, rape lipase also uses a wide range of fatty acids and alcohols as reaction partners. However, the rates of esterification of petroselinic, γ-linolenic, stearidonic and docosahexaenoic acids are only between 2 and 7% that of oleic acid. The common feature of these fatty acids is that the first double bond is cis-4 or cis-6. Petroselaidic acid with a trans-6 double bond is esterified about 10-times faster than petroselinic acid. Arachidonic and eicosapentaenoic acids, both with the first double bond being cis-5, are esterified about 20-times faster than docosahexaenoic acid. By analogy, tripetroselinin and tri-γ-linolenin are hydrolyzed at 14% and 1.5%, respectively, of the rate of triolein hydrolysis. The rape lipase esterifies primary alcohols but cannot esterify secondary and tertiary alcohols. |
| Databáze: | OpenAIRE |
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