Phenotype analysis of lymphocyte subsets in normal human bone marrow
| Autor: | Pamela Clark, David E. Normansell |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Pathology medicine.medical_specialty CD3 Complex medicine.drug_class CD3 CD8 Antigens CD2 Antigens Receptors Antigen T-Cell Bone Marrow Cells Biology Monoclonal antibody Flow cytometry Epitopes Antigen Antigens CD Bone Marrow T-Lymphocyte Subsets mental disorders medicine Humans Receptors Immunologic medicine.diagnostic_test Antibodies Monoclonal General Medicine T lymphocyte Flow Cytometry medicine.anatomical_structure Phenotype Immunology CD4 Antigens biology.protein Bone marrow Antibody psychological phenomena and processes Homing (hematopoietic) |
| Zdroj: | American journal of clinical pathology. 94(5) |
| ISSN: | 0002-9173 |
| Popis: | T-lymphocyte subsets in marrow aspirates taken from normal donors were analyzed with the use of monoclonal antibodies and flow cytometry. Peripheral blood specimens from the same donors were analyzed concurrently and served as controls. Eighty-six percent of CD8-positive cells from marrow expressed the pan-T-cell markers CD2 and CD3, compared with 91% and 81%, respectively, of CD8-positive cells from peripheral blood. Virtually all CD8-positive marrow lymphocytes, however, were negative for CD11b as opposed to peripheral blood, where 18% exhibited positivity for this marker. Further, a higher percentage of marrow CD8-positive T-lymphocytes expressed HLA-DR (15.4%) than did those from peripheral blood (4%). Marrow CD4-positive subsets did not differ substantially from those of peripheral blood. These results support the concept of a selective homing of CD8-positive T-cells to the marrow and indicate that CD8-positive T-cells in the marrow are effector T-cells. |
| Databáze: | OpenAIRE |
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