Efficient delivery of chlorin e6 into ovarian cancer cells with octalysine conjugated superparamagnetic iron oxide nanoparticles for effective photodynamic therapy
| Autor: | Luyi Zheng, Naoki Komatsu, Hongmei Qin, Ikuo Tooyama, Hongkuan Yang, Li Zhao, Shiguang Zhao, Tsukuru Amano, Takashi Murakami, Akimasa Takahashi |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Reactive oxygen species Materials science medicine.medical_treatment Biomedical Engineering Photodynamic therapy 02 engineering and technology General Chemistry General Medicine Conjugated system 021001 nanoscience & nanotechnology 03 medical and health sciences 030104 developmental biology Biochemistry chemistry Cancer cell medicine Zeta potential Biophysics General Materials Science Photosensitizer Nanocarriers 0210 nano-technology Cytotoxicity |
| Zdroj: | Journal of Materials Chemistry B. 4:7741-7748 |
| ISSN: | 2050-7518 2050-750X |
| Popis: | In cancer treatment, efficient delivery of active anticancer drugs into cancer cells is highly desirable for maximizing therapeutic effects and alleviating side effects. In this work, a nanocarrier consisting of an Fe3O4 core, a polyglycerol coating, and an octalysine functionality (SPION-PG-Lys8) has been designed, synthesized and used to deliver a photosensitizer, chlorin e6 (Ce6), into cancer cells for photodynamic therapy (PDT) of cancer cells. SPION-PG-Lys8 is colloidally stable in various aqueous solutions, showing a high positive zeta potential of 47.2 ± 6.9 mV in pure water. In vitro characterization reveals that SPION-PG-Lys8 is efficiently taken up by SKOV3 ovarian cancer cells, exhibiting low cytotoxicity, and suppressed autophagy compared to bare SPIONs. Negatively charged Ce6 is thus loaded on the SPION-PG-Lys8 through electrostatic attraction to yield a SPION-PG-Lys8/Ce6 nanocomplex with a positive zeta potential of 22.4 ± 4.3 mV. SPION-PG-Lys8/Ce6 is more easily taken up by the cells than free Ce6, and surprisingly, the internalized SPION-PG-Lys8/Ce6 is found to be enriched in the mitochondria. SPION-PG-Lys8/Ce6 exhibits almost no cytotoxicity under dark conditions, but strong photocytotoxicity due to the light-triggered production of reactive oxygen species (ROS) destroying the mitochondria. Taken together, our results highlight the great potential of SPION-PG-Lys8 as an efficient carrier of Ce6 for photodynamic cancer therapy. |
| Databáze: | OpenAIRE |
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